Unit A - mitosis/meiosis Flashcards
the cell cycle
process of cell growth leading up to division
interphase
state imbetween cell division, not actively dividing
mitosis
cell division through the nucleaus
-duplication/seperation of chromosomes
-duplication of nuclei
smaller sections of interphase
Gzero, S phase, G2, mitosis
Gzero
cell growth
-nutrient sufficent?
-chromatin
S phase
replication of genetic material (DNA)
-chromatin/chromosomes
s phase stands for
synthesis phase
G2
last minute checks, more growth
phases of mitosis
prophase (first formal step), metaphase, anaphase, telaphase
prophase
-nucleaus condenses
-last half an hour to an hour
-nuclear envelope begins to disappear
-centrioles move to opposite sides of nucleaus
-astral rays form spindal fibers
“preparation for mitosis”
metaphase
-spindle fibers stretch out throughout cell
-line up at metaphase plate
-best time for karyotyping
anaphase
chromisomes pull apart
-spindle fibers pull apart sister chromatids at centromere
telophase
end of mitosis, starts to physically seperate the cells
cytokinesis
creation of a new cell, spliting of cytoplasm
-organelles are all present, and in seperate spots
-seperation forms in the center, sinching/pinching
-produces two daughter cells
apoptosis
programmed cell death
apoptosis in toes/fingers
when humans are being formed, the skin cells between their toes and fingers have to go through apoptosis
sexual reproduction (cellular level)
combination of haploid cells into diploid cell
n+n = 2n
asexual reproducyion (cellular level)
an exact copy of a cell, to create two identical ‘daughter cells’
daughter cell
cells that are the result of a single dividing cell
asexual reproduction is
mitosis
sexual reproduction is
meiosis
diploid
two complete sets of chromosomes
-after fertilization
haploid
single set of chromsomes in an organism
example - egg cells and sperm cells
half of cells (chromosomes)
examples of mitosis reproduction
-bacteria (binary fusion)
-yeast
-spores
importance of sexual reproduction
creates variation (evolutionary advantages)
purpose of cell division
-growth of an organism
-tissue repair/replacement
-reproduction
chromatin
long linear strands of DNA and proteins, de-condensed form of DNA
chromosomes
DNA and associated protiens, densed into thick rods
-more efficent division and transport
histones
protiens on spools of DNA
-sites for DNA to wrap around, easier to transport
-what makes a chromatin, a chromosome
super coiling
wraping around histones to create rod shapes
centromere
point in the middle of an x chromosome
-attachment point for other proteins during cell division
-spindle fibers grab onto
nucleaur envelope
protection for the DNA inside the nucleaus
G1 leads to
-continuing cell cycle, so s phase
-enter Gzero (can be permanent or reversible)
example of permanent Gzero
neurons (most)
reversible quiescence
-upon certain stimulance, cells can re-enter the cycle
example : immune cells
late interphase
-DNA/organelle replication has occured, chromosome form
-centrioles divided, begining to move apart
centrioles
found perpendicular
-important proteins in the cell
astral rays
help to move around centrioles and form spindal fibers (microtubules)
microtubules
major part of cytoskeleton, help maintain shape of cell
spindal fibers purpose in mitosis
grab onto chromosomes to pull them apart
kinetochore region
where the spindle fibers grab onto chromosomes
chromatids
when chromosomes are split, single chromosome
metaphase plate
cells equator, where the spindle fibers pull apart for even splitting
karyotyping
pairing and ordering all chromosomes in an organism
centromere
center of the chromosome
sister chromatid
chromosome in pair format
nondisjunction
if sister chromatids don’t seperate
telophase (early)
-fibers are pulling, the cell begins to stretch
-cell takes on football shape
-where center fiber meets, they push against fibers to continually pull cell apart
telophase (late)
-two seperate ‘packages’ have formed
-nucleaus (two on either side) begins to reform
-spindle fibers disappear
-chromosomes unwind into chromatin
cleavage furow
-middle portion of cell in cytokinisis (where it splits)
microfilaments form contachie ring, which pinches cells into two halfs
difference between plant cell and animal cell
plant cells have cell wall AND cell membrane
-cell organelles near cleavage furrow harden to produce cell walls for daughter cells
golgi purpsoe in the plant cell
brings polysaccharides for cell wall growth
cloning
taking an organisms information putting it into a surrogates organism, then developing the surrogate into a clone
DNA lives in the
nucleaus
somatic
body cell
egg cell donor steps
remove the egg cell, take only the empty cell (get rid of the nucleaus) fuse cell
nucleus donor
take certain cell, remove nucleaus to be infused with empty egg cell
biological definition of clones
identical DNA information
what organism is the clone identical too?
donated nucleaus organism
NOT the surrogate organism
how long can cells live
120 days to 30 years
at what age is there no cells left to divide in a human
115
once cells specialize/differenciate what happens..
thier ability to divide is reduced
what cells ignore the aging rule
spermatocytes and cancer cells
telomeres
‘caps’ on the end of DNA
-space for the end of DNA to fill
-vitalroleinprotectingthedna
what phase does chromatin exist
interphase and late telophase
what phase does chromosome first exist
prophase
when does DNA duplicate itself
interphase
chromatid vs chromatin
chromatid - one side of a identical pair of chromosome
chromatin - unbound spool of DNA that will eventually wind into a chromosome
three main purposes of mitotic cell division
growth, repair, asexual reproduction
what is in the cell during interphase
nucleus, chromatin (duplicated), centrosomes (with pair of centrioles)
what is in the cell during prophase
early mitotic spindle, aster, chromosome (consisting of two sister chromatids),
what is in the cell during metaphase
chromosome, spindle fibers, centrioles. metaphase plate
what is in the cell during anaphase
centrioles, spindle fibers, sister chromasomes in chromatid form
cancer
uncontrolled cell division and growth