Unit 5:The Mouse Flashcards
genus species of mouse
mus musculus (Mus=theif)
organ used in mice to detect pheromones, located in roof of mouth
vomeronasal organ (Jacobsons organ)
what is brown fat
hibernation fat, has increased blood flow for warmth
spot behind eye which we can draw blood from
what is this called in rats?
orbital sinus
rats= retro-orbital plexus
what is barbering
when dominant mouse pulls hair from face and body of other mice
when female mice develop nipples
9 days old
how many pairs of nipples females have
5
why rodents mice commonly get abdominal hernias
have an open inguinal canal which allows the spermatic cord to pass thro
whitten effect
group of females synchronize estrus cycles in presence of male
bruce effect
recently bred females may abort pregnancy when exposed to strange male (usu w/in 4 days of breeding)
what may cause cannibalism of neonates in mice
high-pitched sounds, little nesting material, and if disturbed; you should leave then 2 days undisturbed, preferably a week
what is the “milk spot” on neonate mice
see full stomach on left side of abdomen in healthy mice
how are nude mice produced? what research are they useful in
occur naturally (genetic defect), have deficient immune systems therefore useful in immune studies and cancer research
how to diagnose pinworm
do take prep on perianal region
what causes tyzzers
poor sanitation, overcrowding, stress
why tyzzers is hard to get rid of
produce spores, gram neg
what is Sendai virus
resp infection, compromises immune system, deadly
what is ectromelia
mouse pox, affects v old and v young mice
3 forms of ectromelia
acute- diarrhea, conjunctivitis, swelling of face and extremities, fatal
chronic- cutaneous rash leading to swelling and limb necrosis
Latent- older mice only, harbor in intestinal tract and spread (carriers)
how to do intraperitoneal injection
diff between rat and mouse
anesthetize, scruff in dorsal recumb., point head towards floor, insert needle in lower right quadrant in mice (avoid spleen)
lower left in rats to avoid cecum
why we do not withhold food before anesthetizing mice
fast metabolism, would go in shock
mice (and rats) have __ digits on front feet __ digits on back
4 front, 5 back
difference between galbladder in mice and rats
rats do not have, mice do
mouse lifespan
12-36 months
mouse dental formula
same as rat; I 1/1 C0/0 M3/3
how long mouse gestation, how big are litters?
19-21 days (have ab 6-16 pups)
weaning age in rats and mice
21days (18-21 in mice)
what are hybrid mice
cross of 2 inbred strains
how transgenic mice are created
inject DNA from one animal into fertilized egg of another
what is murine colonic hyperplasia
a gram neg bacteria that causes explosive diarrhea, prolapsed rectum. treat w antibiotics
signs of tyzzers
anorexia, dehydration, diarrhea
how to restrain mice
grab base of tail, scruff, hold tail w pinkie finger
a bubble of fluid under the skin is referred to as what
a bleb
how to give SQ injection on mouse
scruff, allow it to hang onto cage w front paws, insert into tent base parallel to body
how to draw blood from mouse tail
warm mouse, anesthetize, restrain, cut off up to 1 cm of tail tip, collect in hematocrit tube, cauterize or apply pressure
list all blood collection options for mice
tail, jugular, dorsal pedal vein, orbital sinus, facial vein
how to do facial and dorsal pedal vein blood collection
nick vein
how to collect blood from orbital sinus is mice
anesthetize, stick hematocrit tube in lateral canthus at 45 degree angle
most humane euthanasia method
OD of isoflurane (other methods the same as rat methods)
Age mice become sexually mature
6 weeks
