Unit 3b; Disease transmission and control Flashcards
what happens during quarantine period
evaluation of baseline physiological data, vaccinate, monitor for disease, treat disease
what is conditioning (may occur during quarantine or after)
animals exposed to same light, noise, temp, etc, condition that they will be during the study
rodent colonies sometimes use sentinel programs to monitor for disease, what is this?
sentinels are healthy animals placed in an animal room to detect disease (left for several months w animals), they are then euthanized and necropsied (tissue samples sent out)
sentinel tissue samples come back positive, what happens next?
animals in room assumed to be diseased as well, euthanized
microbiological status of animals bred in conventional facilities
carry unknown and uncontrolled variety of microbes
microbiological status of animals bred in germfree (axenic) facilities
harbor no bacteria, viruses, or other microbes., bred in sterile environment, lack normal digestive flora (must be given sterile f/w and supplements to aid in digestion)
define axenic
w/o strangers (germfree breeding)
microbiological status of animals bred in gnotobiotic facility
lack all microbes except normal digestive tract flora
what is an SPF. specific pathogen free animal
free of certain known pathogens
what are cesarean derived animals, why is this a pro compared to regular birth?
derived by C-section to prevent acquisition of pathogens by delivery
what are barrier-sustained animals (what microbiological statuses would this apply to?)
animals maintained in a completely sterile environment
axenic(germfree) and gnotobiotic
what are transgenic mice
genes for traits or susceptibility injected into fertilized eggs, embryos implanted, genes expressed in offspring
what are knockout mice and their importance
genetically engineered mice in which gene(s) have been turned off thro targeted mutation. study role of genes which have been sequenced but whos functions have not been determined.
define biosafety level I
pathogens that ordinarily do not cause disease in humans (animals vaccines everyday products)
define biosafety level II
pathogens that have potential to cause human disease if handled incorrectly (salmonella, toxoplasma)
