Unit 2--Lab Quizzes (4-6) Flashcards
Which of the following microbes causes malaria?
Plasmodium falciparum
Giardia lamblia
Balantidium coli
Trichomonas vaginalis
Plasmodium falciparum
Trichomonas vaginalis differs from many other protozoa because it does not form what structure?
Cell wall
Nucleus
Endospore
Cyst
Cyst
Which mold had asexual spores that appeared like little footballs?
Fusarium racemosum
Penicillium chrysogenum
Rhizopus stolonifer
Syncephalastrum racemosum
Fusarium racemosum
Which of these fungi is “black bread mold”?
Fusarium racemosum
Penicillium chrysogenum
Rhizopus stolonifer
Syncephalastrum racemosum
Rhizopus stolonifer
What does CFU stand for?
Clifton Franklund unleashed
Colony forming units
Cryptic fungus units
Colony
Colony forming units
What is the TDF for Tube A?
0.1 mL —> 9.9 mL (A)
1.0 mL —> 9.0 mL (B)
1.0 mL —> 9.0 mL (C)
1.0 mL —> 9.0 mL (D)
—> 0.1 mL plated
180 colonies
(0.1mL added + 9.9 mL) / 0.1 mL added
= 10 / 0.1
= 100
What is the SDF for tube C?
0.1 mL —> 9.9 mL (A)
1.0 mL —> 9.0 mL (B)
1.0 mL —> 9.0 mL (C)
1.0 mL —> 9.0 mL (D)
—> 0.1 mL plated
180 colonies
Tube A TDF = 100
Tube B TDF =
(1.0 mL added + 9.0 mL) / 1.0 mL added
= 10 / 1.0 = 10
Tube C TDF =
(1.0 mL added + 9.0 mL) / 1.0 mL added
= 10 / 1.0 = 10
SDF = TDF A x TDF B x TDF C
=100 x 10 x 10
= 10,000 (10^4)
How many CFU/mL were in the original flask?
0.1 mL —> 9.9 mL (A)
1.0 mL —> 9.0 mL (B)
1.0 mL —> 9.0 mL (C)
1.0 mL —> 9.0 mL (D)
—> 0.1 mL plated
180 colonies
SDF Tube D =
100 x 10 x 10 x 10
= 100,000 (10^5)
CFU/mL = (SDF x # of colonies) / mL plated
= (100,000 x 180) / 0.1 mL
= 1.8 x 10^8
What is the first phase of bacterial growth?
Lag
Using the points at 120 and 200 minutes, what is the slope (m) of the line?
Time: Absorbance:
120 0.059
200 0.501
m = ( log (Abs 2) - log (Abs 1) ) / (Time 2 - Time 1)
m = ( log (0.501) - log (0.059) ) / (200 - 120)
m = 0.0116
Using the slope (m = 0.0116), what is the generation time (g) of the culture in minutes?
g = log (2) / m
g = log (2) / 0.0116
g = 26.0 min
What is the second phase of bacterial growth?
Log (exponential)
Using the g value (g = 26.0 min), what is the growth rate (k) of the culture in hours^-1?
k (min^-1) = 1 / g
k (hr^-1) = 60 / g
= 60 / 26.0
= 2.31 hr^-1
What term refers to the holes seen in a bacterial culture?
Colonies
Plaques
Voids
Mycelia
Plaques
What term refers to viruses of prokaryotic cells?
Yeast
Viroids
Phage
Prions
Phage
What is the TDF for Tube A?
1.0 mL —> 9.0 mL (A)
1.0 mL —> 9.0 mL (B)
1.0 mL —> 9.0 mL (C)
1.0 mL —> 9.0 mL (D)
—> 10 uL (0.01 mL) plated
23 clear spots (plaques)
(1.0 mL added + 9.0 mL) / 1.0 mL added
= 10 / 1.0
= 10
What is the SDF for Tube C?
Tube A TDF = 10
Tube B TDF =
(1.0 mL added + 9.0 mL) / 1.0 mL added
= 10 / 1.0 = 10
Tube C TDF =
(1.0 mL added + 9.0 mL) / 1.0 mL added
= 10 / 1.0 = 10
SDF = TDF A x TDF B x TDF C
=10 x 10 x 10
= 1,000 (10^3)
What is the best estimate for the original viral titer?
1.0 mL —> 9.0 mL (A)
1.0 mL —> 9.0 mL (B)
1.0 mL —> 9.0 mL (C)
1.0 mL —> 9.0 mL (D)
—> 10 uL (0.01 mL) plated
23 clear spots (plaques)
SDF Tube D =
10 x 10 x 10 x 10
= 10,000 (10^4)
PFU/mL = (SDF x # of plaques) / mL plated
= (10,000 x 23) / 0.01 mL
= 2.3 x 10^7
Which of the following microbes would grow best at pH 5?
Acidophile
Alkalinophile
Psychrophile
Aerobe
Acidophile
Which is the following microbes would grow best at 5º C?
Neutrophile
Psychrophile
Anaerobe
Acidophile
Psychrophile
Which if the following microbes would grow with no added salt and also 5% NaCl?
Acidophile
Psychrophile
Halotolerant
Halophile
Halotolerant
Which of the following microbes would NOT grow with no added salt but would grow with 5% NaCl?
Acidophile
Psychrophile
Halotolerant
Halophile
Halophile
What type of microbial growth is typified by Tube 5?
Tube 5: growth only at the bottom of the tube
Obligate aerobe
Obligate anaerobe
Facultative anaerobe
Aerotolerant
Obligate anaerobe
What type of microbial growth is typified by Tube 6?
Tube 6: more growth at the top of the tube than the bottom
Obligate aerobe
Obligate anaerobe
Facultative anaerobe
Aerotolerant
Facultative anaerobe
