Unit 1-- Lab Quizzes (1-3) Flashcards
What does Dr. Franklund say is the most dangerous thing in our lab?
The microbes
The bunsen burners
The stains
Your lab partner
The bunsen burners
What term refers to the ability to distinguish two objects as being separate?
Magnification
Visualization
Resolution
Granulation
Resolution
When you switch from the 10X to 40X objective, which of these increases?
Working distance
Field of view
Ocular division size
Magnification
Magnification
Which of these microbe categories is the most dangerous that we will use in our lab?
BSL-1
BSL-2
BSL-3
BSL-4
BSL-2
What would the total magnification be if using a 10X ocular and a 25X objective lens?
45X
250X
500X
1,000X
250X
How would the letter “d” appear when viewed through our microscope?
p
b
q
d
p
How to Calculate Ocular Division
(Nonnumbered x 10 um/tick) / numbered
Who created the Gram stain?
Kelsey Grammar
Alexander Graham Bell
Billy Graham
Hans Christian Gram
Hans Christian Gram
What color do Gram negative cells appear?
Pink
Green
Purple
Orange
Pink
What chemical acts as the primary stain in the Gram stain?
Crystal violet
Iodine
Acetone alcohol
Saphranin
Crystal violet
What compound was first used in Koch’s lab to make solid media?
Agar
Gelatin
Cement
Pectin
Agar
Escherichia coli, a Gram negative rod, appeared as purple rods when Gram stained. How do you explain this result?
The stains properly performed
The primary stain was not applied long enough
The mordant was not applied long enough
The decolorized was not applied long enough
The decolorized was not applied long enough
What stain is used in both the Gram and Endospore stain?
Crystal violet
Iodine
Malachite green
Saphranin
Saphranin
What was the mordant for the Endospore stain?
Iodine
Water
Heat
Malachite green
Heat
Which of these was NOT a differential stain?
Gram stain
Capsule stain
Endospore stain
Acid-fast stain
Capsule stain
Which acid-fast staining protocol did we use?
Kinyoun
Ziehl-Nielson
Auramine
Rhodamine
Kinyoun
What is the function of bacterial capsules?
Immune evasion
Carbon storage
Surviving starvation
Motility
Immune evasion
Which is the following bacterial genera most often form endospores?
Clostridium and Bacillus
Salmonella and Escherichia
Myobacterium and Mycoplasma
Staphylococcus and Streptococcus
Clostridium and Bacillus
What did we use to make the background dark in the capsule stain?
Safranin
Iodine
India ink
Malachite green
India ink
What color did acid-fast cells turn in our staining procedure?
Green
Blue
Black
Red
Red
