Pre-Final Bonus Questions Flashcards
Where would you find an Okazaki fragment?
Lagging strand of DNA synthesis
A sushi restaurant
Leading strand of DNA synthesis
Ribosome
Lagging strand of DNA synthesis
Which disease is associated with Entamoeba histolytica?
Dysentery
Toxic shock
Malaria
Vaginitis
Dysentery
Which of these states has the highest incidence of Rocky Mountain Spotted Fever?
Colorado
California
New York
North Carolina
North Carolina
In the Hershey and Chase bacteriophage experiment, which radionuclides entered the cells?
32P
35S
Both 32P and 35S
Neither 32P nor 35S
32P
Which arthropod is the vector for Trypanosoma cruzi?
Kissing bug
Tsetse fly
Deer tick
Mosquito
Kissing bug
What type of immunity is imparted by the RhoGAM vaccine?
Natural passive
Artificial active
Natural active
Artificial passive
Artificial passive
In which of the following type of genetic transfer does the donor survive?
Specialized transduction
Conjugation
Transformation
Generalized transduction
Conjugation
While repeating the work of Messelson and Stahl, you grew Escherichia coli overnight in media with all 15N nitrogen. After pelleting the cells by centrifugation and rinsing, you then allow them to grow for exactly two generation times in media containing only 14N. Which of these patterns will be observed after ultracentrifugation?
All intermediate DNA
Heavy and intermediate DNA
Light and intermediate DNA
Heavy and light DNA
Heavy and intermediate DNA
Where does RNA splicing occur during genetic expression?
Nucleus
Cytoplasm
Ribosome
Nucleoid
Nucleus
What type of genetic regulation is associated with TrpR?
Negative induction
Negative repression
Positive induction
Positive repression
Negative repression
What structure is missing in protozoa cells from the group Excavata?
Ribosomes
Flagella
Nucleus
Mitochondria
Mitochondria
What indicator strain is primarily used to test drinking, waste, and surface water in the United States?
Fecal anaerobes
Fecal streptococci
Fecal bacteriophage
Fecal coliforms
Fecal coliforms
Which of these diseases is associated with ‘rice water’ stools?
Enterohemorrhagic E. coli
Listeriosis
Malaria
Cholera
Cholera
What class of virus has a genome consisting of ssRNA (- strand)?
Class IV
Class III
Class VI
Class V
Class V
What is the primary effect of botulinum food poisoning?
Rash
Lock jaw
Flaccid paralysis
Rigid paralysis
Flaccid paralysis
What is the purpose of primary wastewater treatment?
Reducing the biological oxygen demand
Removal of excess water
Removal of large material
Disinfection
Removal of large material
What term refers to fungi that grow as filaments at room temperature and yeasts at body temperature?
Chimeric
Alternaria
Toxigenic
Dimorphic
Dimorphic
What kind of microbes does a phycologist study?
Algae
Protozoa
Archaea
Fungi
Algae
How do Listeria cells move within the cytoplasm of infected host cells?
Cilia
Axial filaments
Actin polymerization
Flagella
Actin polymerization
What sort of microbe is responsible of Creutzfeldt-Jakob disease?
Bacteria
Prion
Protozoa
Virus
Prion
What is the target of tetracycline’s action?
Mitochondria
Cell wall
Cell membrane
Ribosome
Ribosome
What type of pathogen causes Bancroft’s filariasis?
Fungus
Worm
Protozoa
Virus
Worm
Which of the following nutrient cycles does not involve a gas phase?
Sulfur
Carbon
Phosphorus
Nitrogen
Phosphorus
Who first used a simple microscope live single-celled bacteria?
Leeuwenhoek
Redi
Pasteur
Hooke
Leeuwenhoek
The microbial destruction of concrete sewer pipes is called what?
Biodeterioration
Biofilm formation
Bioremediation
Bioactivation
Biodeterioration
Diphtheria is caused by a toxin encoded by a prophage. What term refers to this process?
Splicing
Lysogenic conversion
Antigenic shift
Conjugation
Lysogenic conversion
What process do most bacteria use to grow and divide?
Schizogony
Meiosis
Mitosis
Binary fission
Binary fission
What genetic sequence does the RNA polymerase bind to during genetic expression?
Shine Dalgarno sequence
Operon
Promoter
Operator
Promoter
Which of the following reactions does the enzyme nitrogenase catalyze?
N2 –> NH4
NH4 –> NO2
NO3 –> NH4
NO2 –> NO3
N2 –> NH4
Which polymerase fills in the gaps after the RNA primers are removed during replication?
DNA polymerase I
DNA polymerase II
DNA polymerase III
RNA polymerase
DNA polymerase I
How are you most likely going to contract tetanus?
Poorly canned food
Ingesting contaminated water
Puncture wound
Inhalation
Puncture wound
What is the first required step for microbial pathogenesis.
Biofilm formation
Internalization
Adherence
Toxin production
Adherence
Which of these microbes is associated with rheumatic fever?
Streptococcus pyogenes
Borrelia burgdorferi
Streptococcus pneumonia
Rickettsia rickettsii
Streptococcus pyogenes
What would happen to bacterial motility if the gene for CheY was knocked out?
The flagella would spin counter-clockwise and the cells would tumble all the time
The flagella would spin clockwise and the cells would run all the time
The flagella would spin counter-clockwise and the cells would run all the time
The flagella would spin clockwise and the cells would tumble all the time
The flagella would spin counter-clockwise and the cells would run all the time
What kind of bacteria contain an O antigen?
Acid-fast
Gram negative
Gram positive
Mycoplasma
Gram negative
Which group of fungi includes the microbe that produces the antibiotic penicillin?
Ascomycetes
Basidiomycetes
Chytridiomycetes
Zygomycetes
Ascomycetes
What are the protein-encoding nucleotide triplets called?
Retrons
Codons
Exons
Introns
Codons
What is the most common type of bacterium on our skin?
Proteobacteria
Actinobacteria
Staphylococcus
Spirochetes
Actinobacteria
What process produces ATP from high-energy intermediates like phosphoenolpyruvate?
Aerobic respiration
Cyclic photophosphorylation
Substrate level phosphorylation
Methanogenesis
Substrate level phosphorylation
Which fungus genus causes Ohio River valley fever?
Blastomyces
Histoplasma
Coccidioides
Cryptococcus
Histoplasma
Which portion of a bacteria endospore contains a thick layer of peptidoglycan?
Core
Coat
Cortex
Exosporium
Cortex
Which of the following must perform reverse electron transport to form NADH?
Green sulfur bacteria
Purple sulfur bacteria
Cyanobacteria
Methanogens
Purple sulfur bacteria
In some models, methanogens appear to promote _______ by reducing the amount of hydrogen available?
Health
Obesity
Diarrhea
Cancer
Obesity
What disease is also known as Hansen’s disease?
Tuberculosis
Typhoid fever
Leprosy
Elephantiasis
Leprosy
What term refers to the distribution of seasonal influenza?
Sporadic
Endemic
Pandemic
Epidemic
Pandemic
What enzyme is used by insertion sequences to replicate and/or move in bacterial genomes?
Transposase
Reverse transcriptase
DNA polymerase
Exonuclease
Transposase
What is the LD50?
Dose of bacteria that kills half of the exposed population
Dose of bacteria that infects half of the exposed population
The time is takes for bacteria to infect half of the exposed population
The time is takes for bacteria to kill half of the exposed population
Dose of bacteria that kills half of the exposed population
Which of these bacteria would contain lipoteichoic acid?
Gram negative
Acid fast
Gram positive
Mycoplasma
Gram positive
What is the best explanation for why Cryptosporidium particularly difficult to control in our water supply?
It is resistant to chlorine
It is resistant to chemotherapeutic drugs
It is resistant to our immune defenses
It is resistant to drying
It is resistant to chlorine
Which portion of our digestive tract has the fewest resident microbes?
Large intestine
Mouth
Small intestine
Stomach
Stomach
What are the NET end products of Glycolysis pathway?
2 pyruvates, 1 NADH, 1NADPH, 2 ATP
2 pyruvates, 1 NADH, 1 NADPH, 1 ATP
2 pyruvates, 2NADH, 2 ATP
2 pyruvates, 2NADH, 1 ATP
2 pyruvates, 2NADH, 2 ATP
Metabolically related microbial populations are called what?
Biofilms
Clones
Guilds
Niches
Guilds
Which of these is true of the archaea membrane?
Contains ester linkages and fatty acid lipids
Contains ester linkages and fatty acid lipids
Contains ester linkages and phytanyl lipids
Contains ether linkages and phytanyl lipids
Contains ether linkages and phytanyl lipids
What process does this reaction correspond to in the nitrogen cycle? NO3 –> N2
Assimilation
Denitrification
Nitrification
Nitrogen fixation
Denitrification
Where would you find the genetic material of a bacterial cell?
Nucleocapsid
Nucleus
Nucleolus
Nucleoid
Nucleoid
Suppose that you serially dilute a culture three times by putting 100µl of culture into 900µl of diluent each time and then transfer 100µl from the third tube onto a plate of media and let it grow overnight. If 15 CFU are observed on the plate, how many microbes were in the original flask?
1.5 x 103 cfu/ml
1.5 x 106 cfu/ml
1.5 x 105 cfu/ml
1.5 x 104 cfu/ml
1.5 x 105 cfu/ml
What term refers to the protein shell that surrounds the viral genome?
Capsule
Envelope
Retron
Capsid
Capsid
Which of the following is NOT an archaea adaptation to growth in high temperatures?
Phospholipid bilayers
DNA positive supercoiling
High internal solute concentrations
Thermosomes
Phospholipid bilayers
Which of these diseases is associated with the Varicella zoster virus?
Mumps
Boils
Shingles
Measles
Shingles
What type of material makes up the TDaP vaccine?
Super antigens
Toxoids
Anti-toxins
Attenuated microbes
Toxoids
Which of the following is NOT an example of an AB exotoxin?
Tetanus toxin
Staphylococcus alpha toxin
Cholera toxin
Diphtheria toxin
Staphylococcus alpha toxin
Which of these is the most common (and least lethal) form of anthrax?
Cutaneous
Sexually transmitted
Pulmonary
Gastrointestinal
Cutaneous
According to modern estimates, how many microbes are in and on our body per human cell?
200
20
2,000
2
2
What process involves the action of autoinducers like homoserine lactones?
Antigenic shift
Phase variation
Attenuation
Quorum sensing
Quorum sensing
During electron transport in Escherichia coli, which of these pairs would pump out the least protons?
NDH-2 and cytochrome bd
NDH-2 and cytochrome bo
NDH-1 and cytochrome bo
NDH-1 and cytochrome bd
NDH-2 and cytochrome bd
What is the incubation period?
The time when disease symptoms are just beginning to appear
The time between getting infected and being able to spread the disease to others
The time between exposure and disease symptoms
The time of the most acute disease symptoms
The time between exposure and disease symptoms
Which of these proteins favors the lytic cycle for bacteriophage lambda?
cII
cro
cI
cIII
cro
Which of these viral classes has a genome that consists of messenger RNA?
Class VI
Class V
Class III
Class IV
Class IV
What term refers to hospital-acquired infections?
Toxigenic
Idiopathic
Nosocomial
Communicable
Nosocomial
What term refers to a persistent disease with a low incidence at a specific location?
Pandemic
Pandemic
Endemic
Sporadic
Endemic
What does R0 best correspond to?
The distribution of a disease in a population
The expected number of secondary infections from each primary case
The resistance of the host cells to infection
The expected number of deaths due to an infection
The expected number of secondary infections from each primary case
What microorganism is responsible for causing whooping cough?
Streptococcus pneumoniae
Corynebacterium diphtheriae
Campylobacter jejuni
Bordetella pertussis
Bordetella pertussis
What would happen to LacZ expression if the gene encoding LacI were knocked out?
Expression would be ON in the presence of glucose and ON in the presence of lactose
Expression would be ON in the presence of glucose and OFF in the presence of lactose
Expression would be OFF in the presence of glucose and ON in the presence of lactose
Expression would be OFF in the presence of glucose and OFF in the presence of lactose
Expression would be ON in the presence of glucose and ON in the presence of lactose
Which of the following diseases is NOT prevented by the TDaP vaccine?
Whooping cough
Diphtheria
Tetanus
Pneumonia
Pneumonia
Which group of microbes predominates in most environments on Earth?
Proteobacteria
Crenarchaeota
Firmicutes
Cyanobacteria
Proteobacteria
Bifidobacterium and Lactobacillus are common examples of what?
Prebiotics
Antibiotics
Probiotics
Abiotics
Probiotics
What disease is associated with Helicobacter pylori?
Diarrhea
Gastric ulcers
Sore throats
Hepatitis
Gastric ulcers
Which of the following cannot carry protons during respiration?
Cytochrome
Flavoprotein
Quinone
NAD
Cytochrome
Ring stage parasites in peripheral blood samples is an indication of which disease?
Toxoplasmosis
Malaria
African sleeping sickness
Filariasis
Malaria
What term refers to people like typhoid Mary, which have an asymptomatic infection of a pathogen?
Fomite
Carrier
Vector
Lysogen
Carrier
Which of these are acellular microbes?
Archaea
Bacteria
Yeast
Phage
Phage
What term refers to the protective effect of having a population of hosts containing many individuals that have developed protective responses against a pathogen?
Mortality
Herd immunity
Attenuation
Morbidity
Herd immunity
Alternate flagella antigen expression due to an invertible promoter region is an example of what?
Antigenic shift
Phase variation
Quorum sensing
Attenuation
Phase variation
Which of these is most commonly acquired by ingesting poorly cooked foods?
Hepatitis A
Hepatitis D
Hepatitis C
Hepatitis B
Hepatitis A
Which of these diseases is NOT spread by the bite of a mosquito?
Malaria
Yellow fever
Lyme disease
West Nile disease
Lyme disease
Which of the following microorganisms is the causative agent of typhoid fever?
Salmonella typhi
Coxiella burnetii
Streptococcus pyogenes
Rickettsia prowazekii
Rickettsia prowazekii
What separates the epilimnion from the hypolimnion in a freshwater ecosystem?
Thermocline
Methane
Neuston
Sediments
Thermocline
Which of these cells is most likely to be affected by HIV?
Epithelial cells
Muscle cells
CD4 helper T-cells
Neurons
CD4 helper T-cells
What pigmented protein do pigmented prototrophic archaea use to collect light energy?
Rhodopsin
Bacteriorhodopsin
Chlorophyll
Bacteriochlorophyll
Bacteriorhodopsin
What term refers to microbes that have decreased in virulence?
Lysogenized
Attenuated
Acellular
Normal microbiota
Attenuated
Which of these is commonly caused by a food intoxication?
Listeriosis
Staphylococcal food poisoning
Cholera
Norovirus disease
Staphylococcal food poisoning
Which of these microbes can lead to severe colitis following long-term antibiotic treatment.
Clostridium difficile
Bacteroides fragilis
Candida albicans
Escherichia coli
Clostridium difficile