Unit 1 : Chapters 1 and 2 Flashcards
protein
strings of amino acids
genes
sections of DNA that code for protein
nucleotides
a collection of neurons within the central nervous system
4 types of nucleotides for DNA
guanine, cytosine, thymine, adenine
- thymine linked with adenine
- guanine linked with cytosine
4 types of nucleotides for RNA
guanine, cytosine, uracil, and adenine
- uracil linked with adenine
- guanine linked with cytosine
DNA and RNA
both consists of a long strand of chemicals called nucleotides
transcription
a single strand of DNA is copied into RNA
translation
mRNA -> protein
- each codon (trio of RNA nucleotides on mRNA copy) codes for on amino acids
- ribosomes read mRNA and spit out proteins based on reading of mRNA codons
southern blots
tissue type : ground up
Purpose : identify IF a particular gene (DNA) is present
northern blots
Tissue type : ground up
Purpose : identify IF and HOW MUCH of a particular mRNA is present
western blots
tissue type : ground up
purpose : identify IF and HOW MUCH of a particular PROTEIN is present
in situ hybridization (ISH)
tissue type : slices
purpose : identify WHERE a particular mRNA is present
immunochemistry
tissue type : sections
purpose : identify WHERE a particular PROTEIN is present
dendrites
one of the extensions of the cell body through which synaptic inputs are recieved
neurons
consist of dendrites, cell body, axon, and axon terminals
cell body
the region of a neuron that is defined by the presence of cell nucleus
-aka soma
axon
a single extension from the nerve cell that carries action potentials from the cell body to other neurons
axon terminals
the end of an axon or axon collateral, which forms a synapse on a neuron or other target cell
process of southern blot
take tissue, grind it up and then analyze it
process of northern blot
take tissue, grind it up, and then analyze it
process of in situ hybridization
take slice of tissue, expose tissue to complimentary string of RNA which is labeled with fluroscent dye to visualize the tissue
process of western blot
take tissue, grind it up, and run the ground up tissue through gel electrophoresis, then transfer proteins from gel to membrane (blotting)
process for immunocytochemistry
take tissue sections and expose sections to antibodies
-antibodies are labeled to enable visualization
gel electrophoresis
uses electric current to seperate proteins by size
glial cells
nonneural brain cells that provide structural, nutritional, and other types of support to the brain
types of glial cells
astrocytes, microglia, schwann cells and oligodendrocytes
are neurons or glial cells larger?
neurons are larger and produce readily measured electrical signals and do most of the work in the brain
astrocytes
removes waste products and cause blood vessels to dilate to bring in more nutrients of times of increased brain activity
microglia
small cells that remove waste, viruses, fungi, and other microorganisms
oligodendrocytes
builds myelin sheaths around certain neurons in the brain and spinal cord (central nervous system)
schwann cells
builds mylein sheaths around certain neurons in the peripheral nervous system
axon collaterals
a branch of an axon from a single neuron