U4 LEC: FIXATION

Question 1

First step in histotechnology

Answer 1

Fixation

Question 2

Fixation is a chemical process by which biological tissues are preserved from?

Answer 2

decay

Question 3

This is a process of preserving cells and tissue constituents in a?

Answer 3

life-like manner

Question 4

T/F: Fixation is done to preserve the original state of tissue before the blood supply was cut off.

Answer 4

TRUE

Question 5

Common specimens in the laboratory

Answer 5

Human tissue from Biopsy or Autopsy

Question 6

Dysfunctional ______ can also be submitted

Answer 6

organs

Question 7

Objectives of Fixation

Answer 7

1. preserve morphological and chemical integrity of cell
2. harden and preserve tissue
3. prevent or arrest degenerative processes

Question 8

Fixation prevents what processes?

Answer 8

• degeneration
• decomposition
• putrefaction
• autolysis

Question 9

What must be seen in the tissue?

Answer 9

nucleus, cytoplasm, membrane (epithelial cells)

Question 10

This chemical is found in certain organs.

Answer 10

Hyaluronic Acid

Question 11

In immunohistochemistry, the membrane is preserved for the diagnosis of?

Answer 11

cancers

Question 12

This process produces decaying tissue.

Answer 12

Autolysis

Question 13

Physical Methods of Fixation

Answer 13

• Heat fixation
• Microwave fixation
• Cryo preservation

Question 14

T/F: Fixation is a largely chemical process.

Answer 14

TRUE

Question 15

Chemical Methods of Fixation

Answer 15

• Immersion Fixation and Perfusion Fixation
• Coagulant fixatives
• Nom-coagulant cross linking fixative

Question 16

Temperature of fixative for Rush Frozen Section

Answer 16

freezing temperature (low temperature)

Question 17

Most commonly used in Fixation

Answer 17

• Formalin
• Formolsaline

Question 18

Fixation involved with agitating the sample in fixative

Answer 18

Perfusion Fixation

Question 19

This clotting factor will stabilize the clot formation.

Answer 19

Factor 13

Question 20

Factor 13 will form what in order to stabilize clot?

Answer 20

cross linking (hardens tissue)

Question 21

This refers to non coagulant cross linking fixatives in which a chemical constituent is taken into the cell, forming molecular complexes and stabilizing proteins.

Answer 21

Additive Fixation / Non-Coagulant Fixatives