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MT6321 HISTOPATH > U4 LEC: FIXATION > Flashcards

U4 LEC: FIXATION Flashcards

1
Q

First step in histotechnology

A

Fixation

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2
Q

Fixation is a chemical process by which biological tissues are preserved from?

A

decay

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3
Q

This is a process of preserving cells and tissue constituents in a?

A

life-like manner

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4
Q

T/F: Fixation is done to preserve the original state of tissue before the blood supply was cut off.

A

TRUE

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5
Q

Common specimens in the laboratory

A

Human tissue from Biopsy or Autopsy

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6
Q

Dysfunctional ______ can also be submitted

A

organs

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7
Q

Objectives of Fixation

A
  1. preserve morphological and chemical integrity of cell
  2. harden and preserve tissue
  3. prevent or arrest degenerative processes
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8
Q

Fixation prevents what processes?

A
  • degeneration
  • decomposition
  • putrefaction
  • autolysis
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9
Q

What must be seen in the tissue?

A

nucleus, cytoplasm, membrane (epithelial cells)

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10
Q

This chemical is found in certain organs.

A

Hyaluronic Acid

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11
Q

In immunohistochemistry, the membrane is preserved for the diagnosis of?

A

cancers

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12
Q

This process produces decaying tissue.

A

Autolysis

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13
Q

Physical Methods of Fixation

A
  • Heat fixation
  • Microwave fixation
  • Cryo preservation
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14
Q

T/F: Fixation is a largely chemical process.

A

TRUE

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15
Q

Chemical Methods of Fixation

A
  • Immersion Fixation and Perfusion Fixation
  • Coagulant fixatives
  • Nom-coagulant cross linking fixative
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16
Q

Temperature of fixative for Rush Frozen Section

A

freezing temperature (low temperature)

17
Q

Most commonly used in Fixation

A
  • Formalin
  • Formolsaline
18
Q

Fixation involved with agitating the sample in fixative

A

Perfusion Fixation

19
Q

This clotting factor will stabilize the clot formation.

A

Factor 13

20
Q

Factor 13 will form what in order to stabilize clot?

A

cross linking (hardens tissue)

21
Q

This refers to non coagulant cross linking fixatives in which a chemical constituent is taken into the cell, forming molecular complexes and stabilizing proteins.

A

Additive Fixation / Non-Coagulant Fixatives

MT6321 HISTOPATH (15 decks)

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