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MT6321 HISTOPATH > U2 LAB: ACCESSIONING > Flashcards

U2 LAB: ACCESSIONING Flashcards

1
Q

under Anatomic Pathology Laboratory Receiving

A
  • Receiving
  • Logging
  • Accessioning/Numbering
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2
Q

under Receiving

A
  • Request form
  • Specimen
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3
Q

Specimen must always be accompanied by?

A

request form

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4
Q

This refers to documenting all steps, all information in request form, and all specimens.

A

Logging

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5
Q

This is the process of giving an identifying marker to a specimen.

A

Accessioning / Numbering

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6
Q

Accessioning starts in the?

A

receiving area

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7
Q

Accessioning is based on?

A

the time it was received

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8
Q

Request form must include:

A
  • Health facility name
  • Hospital number
  • Name of patient*
  • Date/time of request*
  • Age/date of birth*
  • Sex*
  • Address
  • Contact no.
  • Clinical abstract
  • Laboratory investigation/request
  • Specimen
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9
Q

Clinical abstract includes?

A
  • medical history of patient
  • chief complaint
  • details of the patient’s case
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10
Q

Specimen

Routine Histopath

A

Tissue/organ

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11
Q

Specimen

Cytology

A

smears

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12
Q

Specimen

Immunofluorescence

A

renal and skin biopsies

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13
Q

Specimen

fixative for Immunofluorescence

A

saline or liquid nitrogen fixative

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14
Q

Specimen

Immunofluorescence uses this to detect specific locations in the tissue

A

antigens

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15
Q

T/F: Immunofluorescence and Immunostaining uses the same fixative.

A

True

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16
Q

Specimen

Rush Frozen Section

A
  • unfixed specimen
  • specimens for Hirschsprung disease
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17
Q

Specimen

fixative for Rush Frozen Section

A

liquified carbon dioxide or liquid nitrogen

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18
Q

This is used for very high magnifications or ultramicroscopic details of specimen.

A

Electron Microscopy

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19
Q

Specimen Rejection Criteria

A
  • condition is not ideal for test
  • fixative is not sufficient
  • wrong container
  • long transport time
  • wrong or incomplete filled request
  • unlabelled or mislabeled specimen
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20
Q

This refers to decaying processes.

A

putrefication

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21
Q

Process of Logging and Numbering

A
  1. Verify info on request form
  2. Verify Specimen Label
  3. Enter data in logbook/LIS
  4. Accession the specimen
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22
Q

Importance of Accessioning

A
  • avoid mixing/switching
  • track down specimen/results
  • prevents mismanagement
  • process right specimen
  • accurate and timely results
  • proper management/treatment at right time
  • record keeping
  • laboratory planning
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23
Q

First step in histopathologic techniques

A

Accessioning / Numbering

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24
Q

Purpose of Accessioning

A

identify specimen without writing the name of the patient

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25
Q

initial assessment of specimen

A

Accessioning

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26
Q

This is to provide criteria for reception, registration and handling of surgical pathological specimens.

A

Specimen reception, accessioning and handling

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27
Q

All specimens must be delivered to the laboratory within?

A

1 hour maximum

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28
Q

10% neutral buffer takes how many hours for complete fixation?

A

12 to 24 hours

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29
Q

If specimen is within the 1 hour range, put in?

A

automatic tissue processor (to ensure complete fixation)

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30
Q

Patient information in request form

Refers to where the specimen was collected

A

Type of specimen (anatomic site)

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31
Q

T/F: Location of patient must be seen in request form.

A

True

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32
Q

Clinical history of patient must include the following:

A
  • laboratory results
  • pertinent lab works
  • previous biopsies
  • treatment done
  • gynecologic and menstrual history
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33
Q

This is used for pathologic diagnosis.

A

Biopsy

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34
Q

T/F: Surgery residents may sign the request form.

A

True

35
Q

For send out forms to referral laboratories, what must be included?

A
  • Name
  • Address of physician
36
Q

Specimen container must be labeled with the following:

A
  • Full patient’s name
  • Medical record number
  • Age and sex of patient
  • Type of specimen (Anatomic site)
  • Date and time of collection
  • Location of patient (ward, patient ID/bed)
37
Q

If birthday is not written, you may write?

A

medical record number

38
Q

Where should the label in specimen containers be placed?

A

body of container

39
Q

T/F: The one who collects the specimen will be the one to label the container.

A

True

40
Q

Accession Number

S

A

Surgical specimen

41
Q

Accession Number

A

A

Anatomic pathology

42
Q

Accession Number

H

A

Histopathology

43
Q

Accession Number

C

A

Cytology

44
Q

The identity of every specimen is maintained at all time with two identifiers:

A
  • MRN
  • Pathology number
45
Q

Used in identifying the case

A

Case accession number

46
Q

Used if there are multiple specimens for the same patient case

A

Case specimen number (A,B,C)

47
Q

Used if there are multiple blocks on the same specimen

A

Specimen block number (A1, A2, A3)

48
Q

Used if there are multiple slides cut from a single block

A

Block slide number (1,2,3)

49
Q

Refers to specimens that are cut before the physician is asking for a review

A

Recuts

50
Q

All slides other than those stained by H&E will?

A

bear the name of the stain

51
Q

Maintains records of blocks, slides, stains prepared, and is capable of demonstrating volumes for any given time

A

Quadramed

52
Q

This refers to bacterial contamination or bacteria destroying the specimen cells

A

Putrefication

53
Q

Cell is destroyed because of natural process of decomposition or enzymes inside the cell

A

Autolysis

54
Q

This is common in specimens for slide reviews.

A

Damaged specimen or broken slides

55
Q

T/F: The attending physician must be the one to enter the request on the computer.

A

True

56
Q

All specimens from outside the hospital except government must have?

A

approval of Hospital administration and consultant

57
Q

Requests for frozen section require ?

A

24 hours notice ahead of operation (result must be deployed before operation is finished)

58
Q

Accessioning is carried out where and by who?

A

laboratory reception area, technologists

59
Q

Specimen should be immersed in what fixative within 1 hour of biopsy?

A

10% buffered formalin

60
Q

Volume of formalin should be at least _________ volume of the specimen.

A

10 times

61
Q

All specimens must be fixed in 10% formalin except?

A
  • Frozen section
  • Immunofluorescence
  • Electron microscopy
62
Q

unfixed, fresh state

A

Frozen section

63
Q

saline or liquid nitrogen

A

Immunofluorescence

64
Q

2% glutaraldehyde solution in the cold after excision

A

Electron Microscopy

65
Q

Renal biopsies

Before the procedure

A

call 15 minutes, immerse sample in saline

66
Q

Renal biopsies

Examined under the dissecting microscope for?

A

glomeruli
- immunofluorescence
- glutaraldehyde for EM study
- formalin for paraffin processing

67
Q

fixative for Testicular biopsies

A

Bouin’s fluid

68
Q

fixative for Electron microscopy

A

4% glutaraldehyde solution then refrigerate

69
Q

surgical margins should be oriented by sutures

A

Breast Lump

70
Q

The apical nodes should be indicated by sutures.

A

Mastectomy

71
Q

In case of simple mastectomy, this is to be indicated by a separate suture

A

superior margin

72
Q

These are closest lymph nodes to the origin of cancer / malignancy (undergoes metastasis)

A

Sentinel lymph node

73
Q

Requisition for sentinel lymph nodes includes:

A
  • procedure type
  • date and time of injection
  • location of lymph node
  • tracers used
  • radioactivity count
74
Q

If possible, this should be included to ensure integrity of lymph node capsules and assess tumor spread.

A

Perinodal fat

75
Q

Known infective specimen should be clearly marked with?

A

Red marker

76
Q

The proximal or distal excised margins should be oriented by suture.

A

Bowel Resection

77
Q

Received frozen and stored in deep freezer for immunoflurescence studies

A

Skin Biopsies

78
Q

Normally submitted for processing on same day

A

Tiny and small biopsies (Endoscopy and Dermatology)

79
Q

Specimens are usually kept at room temp for how many weeks after the official report is released?

A

2 weeks

80
Q

Referral slides must be accompanies by the official report with following info:

A
  • Patient’s name
  • Medical record number
  • Hospital / clinic pathology number
  • Number of slides / blocks
81
Q

Specimens exempt from pathology examination

A
  • foreign bodies
  • hair, finger/toe nails (cosmetic reason)
  • teeth, dental appliances
  • lens
  • calculi
  • nasal septum cartilage, bone
  • foreskin from circumcision (10y.o. and younger)
82
Q

Specimens accepted for Gross Description only

A
  • torn meniscus
  • varicose veins
  • bone fragments (non-pathologic fracture)
  • ear cartilage
  • fetus
83
Q

This should be performed whenever there is a request by attending physician or at the discretion of the pathologist.

A

Microscopic examination

84
Q

T/F: Specimens for Gross Descriptions are embedded in cassettes.

A

False

MT6321 HISTOPATH (15 decks)

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