U3 LAB: DEHYDRATION Flashcards
Tissue processing involves?
- dehydration
- clearing
- wax infiltration
- embedding
In Rush Frozen Section, this is done simultaneously.
- fixing
- embedding
Pre-eminent type of tissue processing treatment considered to be the most suitable for routine preparation, sectioning, staining, and subsequent storage of large tissue samples.
Paraffin Wax
Paraffin Wax utilizes what as dehydrating fluid?
series of alcohol
This is the removal of water from aqueous fixed tissue.
Dehydration
What step is introduced since most alcohols and paraffin are not miscible?
Clearing
Characteristics of an Ideal Dehydrating Solution
- dehydrate rapidly without producing shrinkage or distortion
- should not evaporate very fast
- should be able to dehydrate even fatty tissues
- should not harden tissues excessively
- should not remove stains
- should not be toxic to the body
- should not be a fire hazard
Characteristics of an Ideal Dehydrating Solution
It should dehydrate rapidly without producing?
considerable shrinkage or distortion of tissues
Characteristics of an Ideal Dehydrating Solution
It should be able to dehydrate even?
fatty tissues
Characteristics of an Ideal Dehydrating Solution
It should not?
- evaporate very fast
- harden tissues excessively
- not be toxic to the body
- be a fire hazard
Commonly used Dehydrating Agents
- Alcohol
- Acetone
- Dioxane
- Cellosolve
- Triethyl phosphate
- Tetrahydrofuran
Dehydrating agents: Alcohol
- Ethanol
- Methanol
- Butanol
Recommended for routine dehydration and considered as the best dehydrating agent
Ethanol
Ethanol is also known as?
Ethyl alcohol
Ethanol
Characteristics
- clear
- colorless
- flammable
Ethanol
Advantages
- fast acting
- mixes with water and many inorganic solvents
- penetrates tissue easily
- not poisonous and expensive
Ethanol
Recommended for?
routine dehydration
Primarily used for blood and tissue films and for smear preparations.
Methanol
Methanol is also known as?
Methyl alcohol
Methanol
Disadvantage
Toxic to the body
Methanol
If introduced to body, what will be affected first?
eyes (blurring of vision)
This is utilized in plant and animal micro-techniques/
Butanol
Butanol is also known as?
Butyl alcohol
Butanol
Advantages
- slow dehydrating agent
- less shrinkage and hardening
Butanol
DIsadvantage
slow dehydrating agent (not suitable for rapid processing)
Increasing alcohol series
60-70% Ethanol > 90-95% Ethanol
99 to 100% Ethanol is also called as?
Absolute Alcohol
Points to remember
The strength of initial alcohol required in each concentration will depend on?
size, nature, fixative
Smaller and more delicate tissues require ______ concentration and _______ intervals between succeeding ascending grades of alcohol.
lower, shorter
These produce shrinkage and make the tissue hard, brittle and difficult to cut.
Concentrated alcohols