U4-3-2 - Thin Layer Chromatography Flashcards
1
Q
A TLC experiment was carried out, but the distances travelled by different spots were all similar.
What adjustment can be made to change this?
A
Use different solvent (or solvent mixture)
2
Q
If two spots of the same compound are run on a TLC plate, their Rf values will be …
A
the same.
3
Q
How many spots will be produced by a pure substance in TLC?
A
One
4
Q
A compound is made and purified. In TLC, it produced two spots.
What does that say about the purity of the compound?
A
Impurities present
5
Q
How can the Rf value be calculated?
A
6
Q
What is the only thing which will affect the Rf value for an individual component?
A
Solvent used
7
Q
How can spots be visualised on a TLC plate?
A
Using a developing agent (e.g. ninhydrin) or UV light
