U4-1-1 - Colorimetry Flashcards
Colorimetry measures how intense the colour of a solution is. The _________ the concentration, the more intense the colour.
Colorimetry measures how intense the colour of a solution is. The higher the concentration, the more intense the colour.
What measurement is taken during colorimetry?
Absorbance of visible light
(or transmittance)
How do you decide which colour filter/light to use in colorimetry?
Must be complementary to the colour of the solution.
(e.g. solution is green = use purple filter/light)
What must be done to the colorimeter before the absorbance of a solution can be measured?
Must be calibrated using a blank
(only solvent in cuvette, usually deionised water)
What is a complementary colour?
Opposite on the colour wheel
Name this piece of equipment
Cuvette
The absorbance measured for a solution gave a concentration higher than any of the standard solutions used to prepare the calibration graph.
What can be done to make the results more accurate?
Dilute the sample
OR
prepare new standard solutions of greater concentration.
To draw a colorimetry calibration graph:
* ____________
* select a suitable filter (complementary colour)
* calibrate colorimeter using a blank
* measure (and plot) absorbance.
To draw a colorimetry calibration graph:
* prepare a set of standard solutions
* select a suitable filter (complementary colour)
* calibrate colorimeter using a blank
* measure (and plot) absorbance.
To draw a colorimetry calibration graph:
* prepare a set of standard solutions
* ____________
* calibrate colorimeter using a blank
* measure (and plot) absorbance.
To draw a colorimetry calibration graph:
* prepare a set of standard solutions
* select a suitable filter (complementary colour)
* calibrate colorimeter using a blank
* measure (and plot) absorbance.
To draw a colorimetry calibration graph:
* prepare a set of standard solutions
* select a suitable filter (complementary colour)
* ____________
* measure (and plot) absorbance.
To draw a colorimetry calibration graph:
* prepare a set of standard solutions
* select a suitable filter (complementary colour)
* calibrate colorimeter using a blank
* measure (and plot) absorbance.
To draw a colorimetry calibration graph:
* prepare a set of standard solutions
* select a suitable filter (complementary colour)
* calibrate colorimeter using a blank
* ____________.
To draw a colorimetry calibration graph:
* prepare a set of standard solutions
* select a suitable filter (complementary colour)
* calibrate colorimeter using a blank
* measure (and plot) absorbance.