translation Flashcards
number of codons that encode for 20 amino a.
64
initiation signal and codes for methionine
AUG
match amino a. to codons in mRNA
tRNA
pairs with the codon on mRNA
anticodon
term for several different codosn that can specifiy an amino acid
wobble base pairing
wobble location
thrid position
covalently couple the amino acid to its appropriate tRNA
aminoacyl-tRNA synthetases
proofreading steps fro aa-TRNA synthesis
selection, editing
process of amino a. binding to synthetase
activation
proteins synthesized in this order
N end to C end
Formation of peptide bond favorable because of this attachment
covalent attachment of tRNA
performs proteins synthesis using more than 50 proteins and several RNA molecules
ribosome
ribosome unit that matches tRNA to codon
small subunit
Ribosome unit that catalyzes peptide bond formation
large unit
Three tRNA binding sites
E (exit), P (peptide bond formation) and A (activating/entrance)
maximum number of binding sites occupied
2
stages of translation
intiation, elongation, termination
Protein finds the place to start protein synthesis
mRNA
loads the initiator tRNA on the small ribosomal unit
eukaryotic initiation factors
the disassociation of this after the AUG start permit the large ribosomal unit to join
initiaition factors
encode for several proteins
polycistronic
start codon sequence for bacteria
Shine-Delgarno
step one of elongation
tRNA bind amino a. to A site
step two of elongation
peptide is released fromm tRNA in P site and binds to A site