Transcription and RNA processing

Question 1

Posttranscriptional processing of Eukaryotic pre-messenger RNA

Answer 1

1. Addition of 7-methylguanosine cap on 5’ end
2. Attachement of a poly-A tail tot he 3’ end

Question 2

RNA is synthesized by

Answer 2

DNA-dependent RNA polymerase

Question 3

tRNA translates in

Answer 3

5’ to 3’ direction

Question 4

What are the two consensus sequences in the bacterial promoter

Answer 4

Pibnow box (TATA box)

-35 sequence

Question 5

snRNA job is

Answer 5

Splicing (removal of introns) mRNA

Question 6

RNA is synthesized in what direction

Answer 6

5’ to 3’

Question 7

Two consensus sequences in eukaryotic promotor are

Answer 7

TATA box (Hogness box)

CAAT box

Question 8

Sigma factor is required for

Answer 8

Initiation of transcription at a promotor

Question 9

RNA polymerase 1 is located in

Answer 9

Nucleolus

Question 10

RNA polymerase 2 is located in

Answer 10

nucleoplasm

Question 11

Activated AA is attached to what part of tRNA

Answer 11

3’ OH region

Question 12

Rho factor is required for

Answer 12

Termination of transcriptions

Question 13

What polymerase synthesizes tRNA, snRNA, and 5s rRNA

Answer 13

RNA polymerase 3

Question 14

RNA polymerase 3 is located in

Answer 14

Nucleoplasm

Question 15

What polymerase synthesizes hnRNA and snRNA

Answer 15

RNA polymerase 2