Regulation of Eukaryotic Gene Expression

Question 1

DNA-binding domains

Answer 1

1. Zinc finers (steriod hormone receptors)
2. Leucine zippers (cAMP-dependent transcription factor)
3. Helix-loop-helix
4. Helix-turn-helix

Question 2

Klein-Waardenburg syndrome

Answer 2

Mutation in PAX gene

1. Dystonia canthorum (lateral displacement of the inner corner of the eye)
2. Pigmentary abnormalities
3. Congenital deafness
4. Limb abnormalities

Question 3

Zellweger Syndrome mutation is what type

Answer 3

Locus heterogeneity involved in peroxisome biogenesis

Question 4

Peroxisome proliferator-activated receptors (PPARs) response element

Answer 4

PPREs

Question 5

Acetylation of histones

Answer 5

Increase gene expression

Question 6

cAMP response element binding (CREB) protein response element (Binding site)

Answer 6

CRE

Question 7

Upstream promoter elements include

Answer 7

CCAAT box that binds a transcription factor NF-1

GC-rich sequence that binds a general transcription factor SP-1

Question 8

Steroid receptor response element (binding site)

Answer 8

HRE