Transcription Flashcards
Region of DNA that includes signals for transcription initiation, elongation, and termination
Transcription unit
TRUE/FALSE: the coding strand is transcribed by RNA.
FALSE. The DNA template strand is transcribed by RNA. The coding or nontemplate strand corresponds with the mRNA transcript except for T’s which become U’s
Starting point of transcription
5’ nucleotide of mRNA
RNA polymerase that generates the primary transcript
RNA Pol II
RNA molecule that carries DNA code from the nucleus to the protein synthesis site
MRNA
Step in transcription wherein RNA polymerase and cofactors bind to sigma-promoter of the DNA
Pre-inititation
TRUE/FALSE: primers are required for transcription
FALSE. RNA transcription has the ability to begin de nuovo.
AT- rich regions which are recognised by the sigma subunit during initiation of transcription
Promoter Sites
Step wherein RNA polymerase leaves the promoter and allows a new polymerase to enter the DNA
Promoter clearance
Subsequent nucleotide triphosphate incorporation in transcription bubbles
Elongation
Terminator sequence in transcription
Rho protein
TRUE/FALSE: error rate is higher in RNA than in DNA synthesis
TRUE. There are no proofreading steps in transcription
Molecules that remove introns from the primary transcripts, leaving exons
Spliceosomes
Molecules vital in guiding RNA polymerase in determining which genes are to be turned on
Transcription Factors
Function of 5’-methylguanosine cap
Recognition of mRNA transcript, protection from phosphatases and nucleases
