Basic Transcriptional Control Mechanisms Flashcards
Proteins or structural motifs that may be produced on one chromosome but act on another
Trans-acting molecules
DNA sequences which influence gene expression on the same chromosome
Cis-acting elements
Genes involved in a linear array regulated by a single promoter
Operon
Smallest unit of genetic expression
Cistron
Conditions for lacI to be repressed in E. coli
High glucose, low lactose
Protein synthesized by lacZ
Beta galactosidase
Protein synthesized by lacY
Lactose permease
Protein synthesized by lacA
Thiogalactoside transacetylase
Binds to Lac repressor and prevents RNA Pol from recognizing Lac operator
Allolactose
Causes attachment of RNA polymerase to promoter region to allow synthesis of beta galactosidase, lactose permease, and thiogalactoside transacetyase
CAP-cAMP
T/F: the histidine operon synthesizes the histidine attenuator only when histidine is already present
TRUE
Pathway preferred for bacteriophage lambda under favorable growth conditions
Lytic growth pathway
T/F: eukaryotic gene expression is polycistronic.
FALSE
Molecule responsible for splicing exons together
Spliceosomes
Region of DNA that includes signals for transcription initiation, elongation, and termination
Transcription Unit