Topic 8 - CELL PHYSIOLOGY II Flashcards
overview
- cells = living ______ _____ of body
- chemical processes __ ____ (performed by cells) = __________
- ___________ = building molecules
- catabolism = __________ of molecules
- cells = living building blocks of body
- chemical processes in body (performed by cells) = metabolism
- anabolism = building molecules
- catabolism = breakdown of molecules
overview
- _____ = ______ building blocks of _____
- _______ ______ in body (______ __ _____) = metabolism
- anabolism = ________ _____
- ________ = breakdown of molecules
- cells = living building blocks of body
-
chemical processes in body (performed by cells) = metabolism
- anabolism = building molecules
- catabolism = breakdown of molecules
anabolism
building molecules
catabolism
breakdown of molecules
overview
no hints
- cells = living building blocks of body
- chemical processes in body (performed by cells) = metabolism
- anabolism = building molecules
- catabolism = breakdown of molecules
Basic Cell Processes
Protein Synthesis
proteins can be???
proteins can be enzymes, structural, etc.
Basic Cell Processes
Protein Synthesis
process of protein synthesis involves what?
- DNA transcription
- Translation
- after translation
Basic Cell Processes
Protein Synthesis
process of protein synthesis involves DNA transcription
DNA ⇒ _________
DNA ⇒ mRNA (messenger RNA)
Basic Cell Processes
Protein Synthesis
step 1
DNA _____ at ____ ___ ____ to expose ____ ____ _________
step 1
DNA uncoils at site of gene to expose gene base sequence
Basic Cell Processes
Protein Synthesis
Step 1
______ uncoils at site of gene __ ______ gene base sequence
step 1
DNA uncoils at site of gene to expose gene base sequence
Basic Cell Processes
Protein Synthesis: DNA Transcription
step 1
no hints
DNA uncoils at site of gene to expose gene base sequence
Basic Cell Processes
Protein Synthesis: DNA Transcription
Step 2
____ ______ is formed _____ code on ____ ______ to add ________ RNA nucleotides
RNA strand is formed using code on DNA template to add complementary RNA nucleotides
Basic Cell Processes
Protein Synthesis: DNA Transcription
Step 2
RNA strand __ _____ using ____ on DNA template __ _____ complementary ____ ______
RNA strand is formed using code on DNA template to add complementary RNA nucleotides
Basic Cell Processes
Protein Synthesis: DNA Transcription
Step 2
no hints
RNA strand is formed using code on DNA template to add complementary RNA nucleotides
DNA transcription
DNA ⇒ mRNA
A ⇒ ?
T ⇒ ?
C ⇔ ?
A ⇒ U
T ⇒ A
C ⇔ G
Basic Cell Processes
Protein Synthesis: Translation
mRNA ⇒ _______
mRNA ⇒ proteins
Basic Cell Processes
Protein Synthesis: Translation
step 1
_____ associates with _________
- __ _____ on mRNA = base _____ = a codon (e.g. CUU, ACG, etc)
- each _____ codes for _ ____ ____ (aa’s can have _____ ______ ______ _____)
mRNA associates with ribosomes
- 3 bases on mRNA = base triplet = a codon (e.g. CUU, ACG, etc)
- each codon codes for 1 amino acid (aa’s can have more than one codon)
Basic Cell Processes
Protein Synthesis: Translation
step 1
mRNA ______ _____ ribosomes
- 3 bases __ _____ = ____ triplet = a ____ (e.g. CUU, ACG, etc)
- ____ codon _____ ___ 1 amino acid (__ ____ ____ more than one codon)
Basic Cell Processes
Protein Synthesis: Translation
step 1
mRNA associates with ribosomes
- 3 bases on mRNA = base triplet = a codon (e.g. CUU, ACG, etc)
- each codon codes for 1 amino acid (aa’s can have more than one codon)
Basic Cell Processes
Protein Synthesis: Translation
step 1
no hints
mRNA associates with ribosomes
- 3 bases on mRNA = base triplet = a codon (e.g. CUU, ACG, etc)
- each codon codes for 1 amino acid (aa’s can have more than one codon)
Basic Cell Processes
Protein Synthesis: Translation
step 2
_____________ with attached __ and ________ (complimentary ______ to codon) binds to ______ ___
transfer RNA (tRNA) with attached aa and anticodon (complimentary bases to codon) binds to matching RNA
Basic Cell Processes
Protein Synthesis: Translation
step 2
transfer RNA (tRNA) ____ ______ aa and anticodon (_________ bases __ ______) ______ to matching RNA
Basic Cell Processes
Protein Synthesis: Translation
step 2
transfer RNA (tRNA) with attached aa and anticodon (complimentary bases to codon) binds to matching RNA
Basic Cell Processes
Protein Synthesis: Translation
step 2
no hints
transfer RNA (tRNA) with attached aa and anticodon (complimentary bases to codon) binds to matching RNA
Basic Cell Processes
Protein Synthesis: Translation
step 3
______ _____ is formed __ ______ aa to growing ______ _____
peptide bond is formed to attach aa to growing peptide chain
Basic Cell Processes
Protein Synthesis: Translation
step 3
peptide bond is ______ to attach __ to ______ peptide chain
peptide bond is formed to attach aa to growing peptide chain
Basic Cell Processes
Protein Synthesis: Translation
step 3
no hints
peptide bond is formed to attach aa to growing peptide chain
Basic Cell Processes
Protein Synthesis
so overall: sequence of DNA bases determines what which determines what for what?
so overall:
sequence of DNA bases determines mRNA sequence ⇒ determines aa sequence for protein
Protein Synthesis: After translation
proteins produced on free ribosomes are released into where?
cytosol or go to nucleus or mitochondria
proteins released into cytosol or go to nucleus or mitochondria are produced where?
on free ribosomes
Protein Synthesis: After Translation
proteins produced by ribosomes on ER (RER) are released into where?
into ER lumen
proteins released into ER lumen are produced where?
ribosomes on ER (RER)
- proteins produced by ribosomes on ER (RER) - released into ER lumen
- modified by?
- transferred to?
- modified by addition of sugars (glycoprotein)
- transferred to golgi complex in vesicles
- proteins produced by ribosomes on ER (RER) - released in lumen
- _________ addition of sugars
- _________ golgi complex in vesicles
- proteins produced by ribosomes on ER (RER) - released in lumen
- modified by addition of sugars (glycoprotein)
- transferred to golgi complex in vesicles
- proteins produced by ribosomes on ER (RER) - released in lumen
- what does the golgi complex do?
- further _______ protein
- _______ modified protein in _______ and sends to __________
- what does the golgi complex do?
golgi complex
- further modifies protein
- packages modified proteins in vesicles and sends to destination
- proteins produced by ribosomes on ER (RER) - released in lumen
- what does the golgi complex do?
- ______ modifies ______
- packages ______ ______ in vesicles and _______ to destination
- what does the golgi complex do?
golgi complex
- further modifies protein
- packages modified proteins in vesicles and sends to destination
- proteins produced by ribosomes on ER (RER) - released in lumen
- what does the golgi complex do?
no hints
- further modifies protein
- packages modified proteins in vesicles and sends to destination
golgi complex packages modified proteins and sends to destination
what destination?
- cell membrane
- secretion
- lysosomes
lysosomes
membrane-bound organelles containing ______ ______ (acid environment)
membrane-bound organelles containing digestive enzymes (acid environment)
what are lysosomes and what do they contain?
membrane-bound organelles containing digestive enzymes (acid environment)
enzymes
names end in _ _ _
ASE
enzymes
- increase what?
- not used up in?
- very sensitive to?
- very ______
- _______ controlled by ______ (proteins) - cell can what?
- increase reaction rates
- not used up in reaction
- very sensitive to pH, temperature
- very specific
- synthesis controlled by cell (proteins) - cell can vary reaction rates
enzymes
list 5 characteristics
- increase reaction rates
- not used up in reaction
- very sensitive to pH, temperature
- very specific
- synthesis controlled by cell (proteins) - cell can vary reaction rates
enzymes
example diagram
