Topic 6: Energy production - Carbohydrates Flashcards
What type of molecules does catabolism break down
amino acids, glucose, fatty acids, alcohol
what intermediate metabolite is formed
acetyl coA
what is acetyl coA further metabolised into
CO2
what are the 4 stages of catabolism
- breakdown of fuel to building blocks for absorption (GI tract). breakage of C-N AND C-O bond, no energy released
- breakdown into intermediates through many pathways (cytosol or mitochondria). this is oxidative (some energy released), C-C bond broken
- krebs cycle in mitochondria. this is oxidative, acetyl coA to C02
- electron transport chain and oxidative phosphorylation, reducing power into ATP
what does oxidative mean
release of reducing power + energy
describe stage 1 of catabolism
- extracellular (GI tract)
- so nutrients are converted into a form that can be taken up by cells (carbs etc too big -> monosaccharides, amino acids etc)
- forms building block molecules which absorbed from GI tract
- no energy released
describe stage 2 of catabolism
- intracellular (cytosolic or mitchondrial)
- many pathways
- building blocks into simpler molecules. many to few molecules
- oxidative so requires coenzymes like NAD+ -> NADH
- some energy released
describe stage 3 of catabolism
- in mitochondria
- cyclic, single pathway - KREBS CYCLE
- oxidative pathway (requires NAD+ and FAD)
- some energy produced as GTP
- acetyl to 2CO2
- produces precursors
describe stage 4 of catabolism
- in mitochondria
- electron transport chain and ATP formed
- NADH and FADH2 re-oxidised
- O2 required
- lots of ATP produced
how much carbs in an adult
1% in comparison of 15% intake
what are the 3 main dietary monosaccharides
glucose
fructose
galactose
what is the concentration of glucose in the blood
5mM
which cells have an absolute requirement for glucose
red blood cells
neutrophils
innermost cells of kidney medulla
lens of the eye
what does CNS use as fuel
glucose
can use ketone bodies if required
how are dietary carbohydrates broken down in stage 1 of catabolsim
saliva - amylase breaks down starch and glycogen -> dextrins
pancrease - amylase breaks down monosacchardies
small intestine - disccharidases break down disaccharides -> monosaccharides
what are the different disaccharidases
lactose
sucrase
pancreatic amylase (alpha 1-4)
isomaltase (alpha 1-6)
why can’t the body break down cellulose
no enzymes to break down beta 1-4 linkages
can not hydrolyse as beta glycosidic bonds are different
how does lactose intolerance occur
Primary lactose deficiency-
absence of lactase persistence allele as mature, can not produce lactase as adults
secondary -
caused by injury to small intestine (gastroenteritis, coeliac disease, crohn’s, ulcerative colitis\0
in infants and adults, can be reversible
congenital -
autosomal recessive defect in lactase gene, cannot digest breast milk
how are monosacharide absorbed into the GI tract in stage 1 of catabolism
- active transport into intestinal epithelial cells by sodium dependent glucose transport 1 (SGLT1)
- passive transport via GLUT2 into bloody supply
- travels in blood to tissues
- glucose taken up in target cells via faciliated diffusion using transport proteins (GLUT1-GLUT5)
what are the different glucose transporters and where are they found (not really need)
GLUT1 - fetal tissues, adult erythroctyes, blood-brain barrier
GLUT2** - kidney, liver, pancreatic beta cells (insulin dependent), small intestine
GLUT3 - neurons, placenta
GLUT4** - adipose tissue, striated muscle
GLUT5 - spermatazoa, intestine
what is the main feature of stage 2 of catabolism
glycolysis
what are the functions of glycolysis in stage 2
aim to breakdown glucose into 2 x3C pyruvate
oxidation of glucose so NAD+ reduced
NADH production (2 for every glucose)
synthesis of ATP (2ATP per glucose, 4 results but 2 used to activate)
what are the features of glycolysis in stage 2
in all tissues which is cytosolic exergonic, oxidative - releases energy with oxidation of substrates no loss of CO2 lactate dehydrogenase is also formed irreversible
what enzymes are involved in glycolysis in stage 2
Hekokinase (glucosekinase in liver)
Phosphofructokinase-1
Pyruvate kinase