Topic 13.1: From Code to Function Flashcards

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1
Q

what does from code to function mean

A

The DNA code needs to be read and then transformed into something functional

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2
Q

what is the central dogma of biology

A

DNA-> RNA -> Protein

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3
Q

how does DNA become RNA

A

transciption

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4
Q

how does RNA become proteins

A

translation

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5
Q

why is regulation of gene expression important

A

for bacteria = to adapt to environment
for human = genes that need to be expressed to make different cell types must be regulated, and important during development

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6
Q

where are genes made

A

nucleus

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7
Q

where are proteins made

A

cytoplasm

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8
Q

how does DNA in nucleus form protein in cytoplasm

A

mRNA out through nuclear pores into cytoplasm

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9
Q

when do you put a 5 prime and 3 prime end

A

when it is single stranded, eg: mRNA

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10
Q

at what direction do you make proteins

A

from N terminus to C terminus

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11
Q

what is transciptipn

A

simple - ‘copy the code’ from nucleotide DNA sequence to nucelotide RNA sequence

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12
Q

what is translation

A

complex - ‘changing the language’

from nucleotide to amino acid

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13
Q

what are the genes in front of the transcription initiation

A

promoter sequences - regulates where the signal for transcription initiation is along the DNA

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14
Q

what are the genes in front of the transcription termination

A

terminator sequences - regulates where the signal for transcription termination is along the DNA

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15
Q

what makes up a gene

A

coding region and the regulatory sequences around it

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16
Q

the promoter is always

A

upstream from transcription intiation

17
Q

the transcription initiation is always

A

upstream from the coding region

18
Q

termination is always

A

downstream from the coding region

19
Q

how many genes make up haemologlobin

A

2 genes, one codes for alpha subunit and one codes for beta subunit

20
Q

one gene codes for

A

one functional unit = sequence of amino acids in a polypeptide or a sequence of nucleotides in an untranslated RNA

21
Q

what types of RNA are present

A

mRNA, rRNA, tRNA, non coding RNA, snRNA (splicing, miRNA (regulation), antisense RNA (complementary copy of mRNA)

22
Q

what is the differences between RNA and DNA

A

RNA had C2 hydroxyl group, DNA had C2 H atom
RNA contains U instead of T
RNA is single stranded (3 and 5 prime end)
RNA can form stemloops where complementary antiparallel strands can form a mini helix
RNA can form complex 3D structures with non-standard base and other nucleotide linkage

23
Q

what is ribosomal RNA

A

most abundant (80% of total RNA)
few kinds, many copies
small and large rRNA’s
highly conserved throughout evolution

24
Q

how many rRNA do prokaryotes have

A

1 small
2 large
eg: 16S

25
Q

how many rRNA do eukaryotes have

A

2 small
2 large
eg: 18S

26
Q

what is tRNA

A

makes up 15% of total RNA
lots of kinds, many copies
small (79 nucleotides long)
each tRNA is dedicated (cognate) to one of 20 amino acids

27
Q

what is mRNA

A

make up 2-5% of total RNA
lots and lots of kind, few copies
called ‘mRNA transcipts’