Topic 3 Wood Flashcards
What plant was used to find the role of the nucleus?
Acetabularia (mermaid cups)
Features of Acetabularia
has a cap (hat), stalk (stem) and a foot (rhizoid)
What role does a group of genes called a homeobox genes play?
It’s an important role in the development of organisms
Process of differential gene expression
- All cells have the full set of genes
- Different genes are active in different cells
- Only active genes are transcribed to produce mRNA (the transcriptome)
- mRNA from the active genes is translated to produce proteins
- The set of proteins (and enzymes) made by the cell (proteome) control the structure and function of a cell
Cell in differential gene expression
proteome
What does the epigenome influence?
which genes are transcribed in a cell
Histone modification
the binding of epigenetic markers to histone ‘tails’ alters how tightly DNA is wound around the histone proteins
DNA methylation
Methyl group attaches to DNA; prevents transcription
What needs to happen to DNA to be transcribed?
-DNA needs to unwind –> must be accessible for transcription and to make proteins
Repressor molecules
methyl groups –> molecule that prevents transcription
What can enzymes or chemical stimulus’ used for?
to remove methyl groups (blockers) that block RNA from transcribing the gene
What happens when E-coli is left with glucose and lactose?
it needs to respire but is lazy so will use glucose first then it will need an enzyme to break down lactose into glucose and galactose
Explain the results of beta-galactosidase reaction
- If lactose is not present, a repressor molecule binds to DNA which prevents transcription
- If lactose is present the repressor molecule can’t bind to DNA because it will bind to lactose instead
- The DNA is used to produce mRNA, which goes to the ribosome and results in the production of the enzyme beta-galactosidase
Name a plant organ
Leaf
Name a plant organ system
Root system, shoot system
Name a plant tissue
spongy mesophyll
Transcription summary
- Occurs in the nucleus
- The double helix unwinds as hydrogen bonds break between the bases
- The enzyme RNA polymerase attaches to the DNA
- The copy of the DNA strand created is called mRNA
- This molecule then leaves the nucleus through a nuclear pore and attaches to a ribosome
Translation summary
- An anticodon on the tRNA binds to the codon on the mRNA
- Each tRNA molecule has a specific amino acid attached to it
- Peptide bonds join the amino acids to make a polypeptide