Topic 2 Flashcards
What size are bacteria?
Small, they are the most important
What size are viruses?
They are even smaller than bacteria
What is an example of multicellular parasites?
Tapeworms
What can fungi cause?
Some skin infections
What are protists
Single cells eukaryotic organisms
What are prions
Proteins, not living things, will bing to some proteins (in a human) and stop them from working
Which organism has a “true” nucleus?
A eukaryotic (animal) cell
Are viruses alive?
They reproduce (can’t do it alone), don’t have metabolism, don’t take in food, don’t have waste, don’t take up energy, have genetic material so they are not ‘technically’ alive
What is included in Prokaryotic cells?
Bacteria and archaea
What is included in Eukaryotic cells
Protists
Algae
Fungi
Animals
What is included in acellular
Viruses
Prions
What does acellular mean
Without cells
Give an example of a protist
Seaweed
Eukaryotic are what type of cells
Single cells, what humans are made of
Describe the chemistry of Archaea
The chemistry and metabolism is similar to eukaryotic cells, do not cause infection
What are the top 3 groups in Eukarya
Plants, animals (humans), and fungi
What is the chromosome
How DNA is stored
What is the capsule layer?
It is resistant the immune system (more dangerous to humans)
What is the plasmid
It is useful because we can take DNA and put it in a plasmid and trick it to multiple.
It carries a gene that makes bacteria resistant to antibiotics which is not done by eukaryotes
State the features of a Prokaryote cell
Chromosomes: circular - no nucleus - no histones Plus additional small circles of DNA called plasmids Organelles: No Cell wall: Peptidoglycan (most) Cell Division: Binary Fission
What are histones
Positively charged proteins in eukaryote (used for compacted DNA)
State the features of a Eukaryote cell?
Chromosomes: Paired chromosomes, in nuclear membrane
(Histones - proteins used to package DNA into chromosomes)
Organelles: Yes
Cell wall: Polysaccharide (if any)
Cell division: Mitosis
What are the three domains of life?
Eukarya, Archaea, and Bacteria
Do Archaea cause any issues?
No, they do not
What is classification?
Organisms are sorted in a hierarchy (species, genus, family, etc). This system is known as taxonomy
We classify by looking for similar shared traits (which ideally indicate relatedness)
What characteristic do mammals share?
They produce milk
What characteristic do carnivora share?
Specialized teeth
What characteristic do Felidae share?
Retractable claws
What is a species?
A population whose members can interbreed to produce viable, fertile offspring
What are problems with the definition of a species?
- Many organisms don’t mate (there are no sexes)
- Bacteria often swap DNA between unrelated forms
- Viruses can’t even reproduce on their own
What is an example of a mammal that breed but cannot have offspring
A mule (horse and donkey)
How do we classify microbes?
- Cell shape (morphology)
- Ability to be stained
- Biochemistry (nutrition, metabolism & growth conditions)
- rRNA sequences (2-3% different = new species)
Why do we test enzymes
To see what kind of species these microbes are
What is a sequence alignment
Comparing rRNA genes between organisms to see how related they are
How do we name things?
- Use latin (no favouritism)
- Italicized or underlined
- Each organism has two names: Genus and species
eg: Escherichia coli (E.Coli)
Deinococcus Radiodurans is known for being
The “toughest bacterium”
It can survive cold, dehydration, vacuum, high acidity and radiation
What qualities do protistas have?
Animal and plant like qualities (do not fit into the other three kingdoms)
Prokaryotes - Archaea (many are)
Extremophiles and Methanogens
Prokaryotes - Archaea compete with
Bacteria but bacteria do a better job at dividing and growing
Where do you find prokaryotes (archaea)
- Appear in warm climates where they do not need to compete with bacteria
- Can live in water that is boiling
- Can live really deep in the earth crust
- Not known to cause any diseases
Describe the structure of prokaryotes (Bacteria)
- ‘Simple’ single celled organisms
- No nucleus or internal organelles
- Most posses a cell wall containing peptidoglycan
What is endosymbiosis?
One cell living inside of another cell
Give an example of endosymbiosis
Chlamydia lives inside of a cell and does not perform ATP, it steals energy just like parasites
What is the Endosymbiotic Theory
Fusion or symbiotic association of prokaryotic cells
- Ancestors of mitochondria were aerobic heterotrophic bacteria
- Ancestors of chloroplasts were photosynthetic cyanobacteria
Name the structure within a mitochondria
- Outer membrane
- Inner membrane
- Intermembrane space
- Cristae
- Matrix
What type of bacteria are mitochondria?
Aerobic bacteria
What type of bacteria are chloroplasts?
Cyanobacteria
Give an example of photosynthesis
A tree takes in CO2 from the air and releases oxygen?
Give an example of absorption
A fungi, digestion starts outside of the body and then they soak things up
Give an example of ingestion
Amoeba?
Describe Eukaryotes
- Have a nucleus
- Single-celled and multi-cellular organisms
- Classified based on structure, reproductive cycles and how they feed themselves
Describe Animalia
- Multicellular
- Ingest nutrients
- Extracellular matrix
- Sponges?
- Vertebrates and invertebrates
Describe plantae
- Multicellular
- Photosynthetic
- Cellulose
- Ex: mosses, ferns, conifers, flowering plants
Describe Fungi
- Absorb nutrients
- Unicellular/multicellular
- Chitin
- Ex: Yeasts , moulds, mushrooms
Describe protists
- Unicellular organisms
- Don’t fit anywhere else!
Describe the matrix and cell wall of a Eukaryotes
They have an extracellular matrix and no cell wall
What is diploid?
Two sets of information
Describe the stage of animals
Only the diploid stage is multicellular
A gamete is ___ (cell)
Unicellular
Describe the fungi stage
The haploid stage is multi-cellular, the diploid stage consists of a single cell
There may also be a multicellular dikaryotic stage
(Some of the cells in the fungi have 2 nuclei and are haploid)
Where does meosis occur?
In mushrooms and it produces spores?
Describe the sex (Re)Education in plants
“Alternation of Generations”
There are multicellular diploid AND haploid stages
Describe Eukaryotes - Animalia
- Multicellular
- ECM? instead of cell wall
- Most are ingestive
Describe Eukaryotes - Plantae
- Multicellular
- Cell walls made of cellulose
- Photosynthetic
- No known pathogenic forms
Describe Eukaryotes - Fungi
- Single celled or multicellular
- Cell walls containing chitin
- Absorptive
Describe Eukaryotes - Protista
Complex (eukaryotic) single cells that can’t be easily categorized
What type of group are the protista?
A very diverse group of single-celled microbes whose relationships are poorly understood
How are protists grouped?
According to means of nourishment, sex, and ___?
What are the different types of protists
Ingestive protists: Protozoa
Absorptive protists: Water moulds
Photosynthetic protists: Algae
Viruses and Prions have 4 possibilities
Single stranded RNA, DNA, or double stranded RNA, DNA
What are some criteria that may be used for classifying microbes? (4)
- Cell shapes
- Ability to be stained
- Biochemistry or metabolic abilities
- DNA sequence - particularly those for rRNAs
Define the characteristics of a Prokaryotes - Bacteria
- ‘Simple’ single celled organisms
- No nucleus
- No internal organelles
- Most possess a cell wall containing peptidoglycan
Define the characteristics of Eukaryotes - Animalia
- Multicellular
- Made up of Eukaryotic cells
- No cell walls
Define the characteristics of Fungi
- Eukaryotic
- Unicellular
- Multicellular
- Cell walls containing chitin
Define the characteristics of Protista
- Complex (eukaryotic) single cells that can’t be easily categorized
Viruses and reproduction
They cannot reproduce on their own
Define taxonomy
The science of classifying and naming organisms
Define Prokaryote
Any unicellular microorganism that lacks a nucleus. Classificiation include Bacteria and Archae.
Define Eukaryote
Any organism made up of cells containing a nucleus composed of genetic material surrounded by a distinct membrane.
Classification includes animals, plants, fungi, and protozoa
Define Bacteria
Prokaryotic microorganisms typically having cell walls composed of peptidoglycan
Define Fungi
Eukaryotic organisms that have cell walls and obtain food from other organisms
Define Virus
Tiny infectious acellular agent ____?
What are the characteristics of life (8)
- Reproduction
- They have genetic material of some sort (DNA)
- They grow
- They have some sort of metabolism (to utilize energy)
- Maintain homeostasis
- Respond to stimuli
- They adapt very quickly
- They fight entrophy (the tendency towards disorder)
What are the 2 main types of cells?
Prokaryotic and Eukaryotic cells
What are the 3 domains of life?
Eukarya, Archae, and Bacteria
Why are Archae and Bacteria both Prokaryotes?
Because they don’t have nuclei
Describe the structure of a Prokaryotic cell
Size: smaller than Eukaryotic cell Nucleus: No Membrane-Bound Organelles: No Ribosomes: Yes Presence and Type of Cell: Yes, there is a cell wall made of Peptidoglycan
Describe the structure of a Prokaryotic cell
Size: smaller than Eukaryotic cell Nucleus: No Membrane-Bound Organelles: No Ribosomes: Yes Presence and Type of Cell: Yes, there is a cell wall made of Peptidoglycan (some Prokaryotes have lost their cell wall. They may have a capsule/slime layer)
Describe the structure of a Eukaryotic cell
Size: Bigger than Prokaryotic Nucleus: Yes Membrane-Bound Organelles: Yes Ribosomes: Yes Presence and Type of Cell: May have a cell wall
What is the cell wall in plants made of?
Cellulose
What is the cell wall of animals made of?
They do not have a cell wall. We have proteins, known as the extracellular matrix
What types of characteristics do taxonomists use to classify microbes?
- cell structure (shape)
- cellular metabolism
- differences in cell components such as DNA, growth form (how they stick together in chains Ex: Strepto)
- Colour of the colonies
How are rRNA genes used?
rRNA genes are compared to see how similar/different two organisms are