Toll-like Receptors/Roles Flashcards
What does TLR-2 and TLR-6 see?
Extracellular pathogens
Heterodimer
Plasma membrane
Lipoteichoic acid/Gram-Positive
Zymosan
What does TLR-4 see?
Extracelluar pathogen
Plasma membrane
Homodimer
MD2
LPS/Gram Negative
What does TLR-5 see?
Extracellular
Bacterial flagellin
What TLR-3 see?
Intracellular pathogen
DSRNA
What does TLR-7 see?
Intracellular pathogen
SSRNA
What does TLR-8 see?
Intracellular pathogen
SSRNA
What does TLR-9 see?
Intracellular pathogen
Unmethylated CpG rich DNA
What do TLR-1 and TLR-2 see?
Extracellular pathogens
Plasma membrane
Heterodimer
Bacterial lipopeptieds
Explain the TLR cascade?
Ligand binds
Myd 88 adaptor protein
Myeloid differentiation primary response gene= TRL 1,2,5,6,7,9
TLR-4 can activate both pathway
TLR-3 uses TRIf to active IRF
IRAK family
Interleukin-1 Receptor Associated Kinase
TRAF6
TNF Receptor Associated Factor
Activated NF-KappaB
Explain TLR-4 LPS Signaling.
Complex TLR4, MD2, CD14, and LPS at Mac surface
MyD88 binds TLR4 and actives IRAK4
Phosphorylates TRAF6
Phosphorylation of IKk/IKK phoshorylates IKB= degradation
Releases NFKB= inflammatory genes transcribed
What HMGB1?
DAMP= Damage Associated Molecular Patterns
High mobility group box 1
Protein passively released during necrosis
Activates NF-Kappa B
Rage is receptor=Receptor of Advanced Glycation of End Products
What is Uric acid in the sense of immune signaling?
DAMP
Activates NF-Kappa B
What HSP?
Induce NF-Kappa B
Release TNF-Alph and IL-1Beta
Inflammatory
