test 4 lec 23 Flashcards

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1
Q

what bacteria uses more than one type of metabolism

A

rhodopseudomonas palustris

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2
Q

delta pH is what

A

the H+ concentration difference

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3
Q

what is photoheterotrophs

A

absorb light to make ATP, while incorporating pre-formed carbon compounds for biosynthesis

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4
Q

where is the ETS located

A

in a membrane that separates two aqueous compartments

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5
Q

what are great electron acceptors

A

H2O and O2

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6
Q

what is a proton motive force

A

stores energy to make ATP

drives protons across the membrane

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7
Q

what are the three major classes of prokaryotic energy acquiring processes using an ETS

A

organotrophy
lithotrophy
phototrophy

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8
Q

how else is electrical potential and pH difference affected

A

includes charges on other ions

metabolic generation of acids or pH changes outside the cell

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9
Q

how do microbes transfer energy

A

by moving electrons

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10
Q

what is photoautotrophs

A

couple photolysis to CO2 fixation for biomass production

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11
Q

what are obligate autotrophs

A

fix CO2 for biosynthesis

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12
Q

besides ATP synthesis what drive many cell processes

A

delta p

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13
Q

what are facultative lithotrophs

A

chemooganotrophs that catabolize many substrates

oxidizing sulfur compounds

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14
Q

where is energy stored when protons are pumped across the membrane

A

electrical potential and the pH difference

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15
Q

what is the proton motive force

A

the sequential transfer of electrons from one ETS protein to the next yields energy to pump ions across the membrane

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16
Q

ETS proteins and cofactors act as…

A

electron donors and acceptors, coupled with proton transporting across membrane

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17
Q

function of proton potential

A

stores energy to drive H+ back across the membrane through devices such as ATP synthase

18
Q

what is phototrophy

A

involves light capture by chlorophyll, usually coupled by splitting of H2S or H2O or organic molecules

19
Q

function of ETS

A

transfers electrons onto membrane embedded carriers in a series of increasing reduction potential , ending at a terminal electron acceptor

20
Q

a more positive E’ means that

A

that reducing the electron acceptor yields more energy

21
Q

what is the chemiomotic theory

A

energy from electron transfer between membrane proteins is used to pump protons across the membrane, accumulating a higher H+ concentration in the compartment outside

22
Q

what does a negative E equal

A

energy is yielded by the reverse reaction

23
Q

what is the reduction potential

A

a measure of the tendency of a molecule to accept electrons

24
Q

how do electrons move

A

from reduced electron donors to oxidized electron acceptors

25
Q

what is lithotrophy

A

involves inorganic electron donor donors and inorganic or organic terminal acceptors

26
Q

what are chemolithoautotrophs

A

obligate autotrophs, fixing CO2 for biosynthesis

27
Q

a gain of electrons yields what

A

energy

28
Q

what is the proton potential

A

the H+ concentration difference plus the charge differen

29
Q

a more negative E’ means that

A

oxidizing the electron donor yields more energy

30
Q

how does ETS convert its energy

A

ion potential or electrochemical potential between two compartments separated by the membrane

31
Q

the ETS must accept electrons from

A

an initial electron donor consisting of an organic molecule or a reduced mineral

32
Q

the standard reduction potential represents what

A

the standard reduction potential per electron

33
Q

what is organotrophy

A

involves organic electron donors and inorganic or organic terminal electron acceptor

34
Q

oxidoreductases consist of

A

multiple protein complexes that include cytochromes and non cytochromes proteins

35
Q

what happens if the membrane is disrupted so that protons leak

A

small leak can dissipate all the energy store as delta pH

uncouple electron transport from ATP synthesis through the membrane ATP synthase

36
Q

what is a redox couple

A

the oxidized and reduced states of a compound

37
Q

what are oxidoreductases

A

electron transport proteins

oxidize one substrate and reduce another

38
Q

what are standard reduction potentials

A

standard values of E

39
Q

positive E is equal to what Delta G

A

neg

40
Q

characterisitcs of rhodopseudomonas palustris

A

gram neg
purple nonsulfur photosynthetic bacterium
photolithoheterotroph
found in soil and water
in O2, use photosynthesis or lithotrophy
no O2, eats everything to obtain reduced electron carriers

41
Q

a complete redox reaction combines

A

two redox couples
one accepting electrons
and one donating electrons