Test 2- S3 Flashcards

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1
Q

What decides the shape and function of a protein

A

The sequence of amino acids that built it

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2
Q

What are the functions of proteins

A

Structure, enzymes, hormones, antibodies and receptors

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3
Q

What are enzymes

A

Biological catalysts that are produced in all living cells

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4
Q

What is a degradation reaction

A

A reaction in which one substance is broken down into two or more substances.

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5
Q

What is a synthesis Reaction

A

A reaction in which two or more substances are combined to form one substance.

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6
Q

What is the use of enzymes

A

They increase the speed of a chemical reaction without being used up in the process.

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7
Q

How could you describe an enzyme

A

Specific. It only works with one substrate as they have complementary active sites

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8
Q

What do enzymes do

A

They turn substrates into products

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9
Q

How are enzymes involved in synthesis reactions and give an example of one.

A

Enzymes increase the speed of the reaction. An example of a synthesis Reaction is g1pps (glucose-1-phosphate being turned into starch using phosphorylase)

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10
Q

How are enzymes involved in degradation reactions and give an example of one.

A

They increase the speed of the reaction. An example of this is HPCOW (hydrogen peroxide being broken down into oxygen and water using catalase).

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11
Q

What is an enzymes optimum temperature or pH

A

It is the temperature or pH that an enzyme activity is at its highest.

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12
Q

What happens if an enzyme is placed in too high a temperature or a pH out of its range.

A

It becomes denatured and no longer works on its substrate as the shape of the active site changes and it is no longer complementary to the substrate.

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13
Q

What is genetic engineering

A

The artificial transfer of genetic information from one cell to another.

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14
Q

Why are bacterial cells often the recipients of genetic engineering.

A

As they reproduce quickly which means large amounts of substances can be gathered in a short time.

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15
Q

What is the first stage of genetic engineering

A

Identifying the section of DNA that contains the required gene.

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16
Q

What is the second stage of genetic engineering

A

They extract the gene from the chromosome using enzyme ‘scissors’.

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17
Q

What is the third stage of genetic engineering

A

They extract a bacterial plasmid from the target cell and cut a section out suing the enzyme ‘scissors’

18
Q

What is the fourth stage of genetic engineering

A

They insert the already cut out gene into the plasmid

19
Q

What is the fifth stage of genetic engineering

A

The plasmid is then returned to its host cell to produce the GMO

20
Q

What is the final stage of genetic engineering

A

The GMO is placed in ideal condition where it reproduces quickly, this can then be isolated and extracted for use.

21
Q

How are enzymes used in the process of genetic engineering

A

The enzymes are used as scissors to cut parts out of plasmids and chromosomes.

22
Q

Give an example of a GMO

A

Human insulin is grown in bacterial cells to quickly reproduce them to treat human diabetes. Other examples include: tomatoes with longer shelf life and birds that are resistant to bird flu.

23
Q

What is respiration

A

The way that cells break down glucose into energy

24
Q

How could you describe the reaction of respiration

A

It is enzyme controlled

25
Q

What is the energy released by respiration is used to do what

A

To generate ATP

26
Q

What can the energy transferred by ATP be used to do.

A

Cell division, muscle cell contraction, protein synthesis, active transport within cells and nerve impulses.

27
Q

(Stage 1 Respiration)

Where does stage one occur and what does it require

A

The cytoplasm and it doesn’t require oxygen

28
Q

(Stage 1 Respiration)

What occurs during the first stage

A

A glucose molecule is broken down into two pyruvate molecules.

29
Q

(Stage 1 Respiration)

What can the energy yielded by this degradation do.

A

It is used to generate 2 molecules of ATP using 2ADP and 2Pi

30
Q

(Stage 2 Aerobic Respiration)

Where does this stage occur and what does it need

A

It occurs in the mitochondria in aerobic conditions and oxygen is present/needed.

31
Q

(Stage 2 Aerobic Respiration)

When does this stage occur.

A

It only occurs after stage 1 if oxygen is present. If not, fermentation occurs.

32
Q

(Stage 2 Aerobic Respiration)

What occurs in this stage

A

In this stage each pyruvate molecule from stage one is broken down into carbon dioxide and water.

33
Q

(Stage 2 Aerobic Respiration)

What can the energy yielded do

A

It can produce 36 molecules of ATP from 36ADP and 36Pi

34
Q

(Stage 2 Aerobic Respiration)

Overall, how many molecule of ATP are produced from stage 1 and 2.

A

38

35
Q

-Fermentation(Stage 2 if no oxygen)-

What is fermentation.

A

Fermentation is the breakdown of glucose in the absence of oxygen.

36
Q

-Fermentation(Stage 2 if no oxygen)-

What is the pyruvate converted into in plant cells

A

Ethanol and CO2

37
Q

-Fermentation(Stage 2 if no oxygen)-

What is the pyruvate converted into in animal cells

A

Lactate (lactic acid)

38
Q

-Fermentation(Stage 2 if no oxygen)-

What can these two Reaction be described as

A

Irreversible

39
Q

-Fermentation(Stage 2 if no oxygen)-

What can the energy produced in these two Reaction be used to do.

A

Make 2 molecules of ATP from 2ADP and 2Pi.

40
Q

Where does fermentation occur and how effective is it

A

In the cytoplasm and it is much less effective/ efficient than respiration.