Higher Assignment Flashcards
What is your assignment’s title?
The effect of inhibition on enzyme activity
What is the aim of your assignment?
To investigate the effect of sodium chloride concentration on enzyme activity
Point to mention in paragraph 1 of underlying Biology (enzyme action)
1: brief summary of enzyme action and specificity
2: describe substrate affinity
3: describe induced fit and resultant orientation
4: results of induced fit, such as deceased activation energy
Points to mention in paragraph 2 of underlying biology (inhibition)
1: description of general inhibition
2: name two main types of inhibition
3: describe competitive inhibition
4: describe non-competitive inhibition
5: explain why competitive is reversible (and how), whilst non-competitive isn’t.
Name all required details in your method:
a) chemicals
b) apparatus
c) measurements
a) varying concentration of sodium chloride solution, catalase solution and hydrogen peroxide solution
b) Eppendorf tubes, beaker, water bath, syringe, paper disc and stopwatch
c) time taken for disc to rise, volumes of each chemical
What must you do with your internet source?
Number it with a figure and then reference this figure at the end, with the source’s URL. DO NOT WRITE THE URL UNDERNEATH THE SOURCE
What type of graph will you use?
A line graph
What will your… be:
a) x-axis scale and label
b) y-axis scale and label
a) sodium chloride concentration; 0, 0.1, 0.2, 0.3, 0.4
b) time taken for disc to rise to surface; 15 to 30 in increments of 1
What will you include in your analysis?
Percentage increase of time taken, between concentrations of 0 and 0.4
What is your percentage increase?
87%
What will you then state after calculating percentage increase, in your analysis?
This demonstrates the increased time taken for the disc to rise, as the sodium chloride concentration increased within each Eppendorf tube - equating to a progressively decreased enzyme activity. This decrease in activity is similarly shown in figure 1.
What is your assignment’s conclusion?
Overall, as the concentration of sodium chloride increases, enzyme activity decreases. This can be seen through the trend of the operational dependant variable, as the time taken for each disc to rise increased significantly as the sodium chloride concentration increased - equating to a decreased activity. This is shown in figure 1, as the graph’s downward trend indicates the steady decrease in enzyme activity as the sodium chloride (NaCl) concentration decreased
What are your points of evaluation?
1) increased validity, due to the use of a control water group.
2) reliability was increased through the process of repeated measurements, before gaining an average.
3) validity was improved to a further extent through the use of water baths. However, validity was decreased by the absence of a pH buffer.
What is the significance of a control group?
Allows comparison with other groups, during the experiment, as this group produces abnormal results compared to others. For example, the use of a control group of water allowed the conclusion that sodium chloride concentration was the driving factor behind this variation in time taken for discs to rise, as the control group’s disc was the quickest to do so - equating to the greatest enzyme activity.
What is the significance of repeating measurements to gain an average?
This repetition and mean calculation allowed the effect of certain anomalies to be reduced, within individuals results, preventing the overall trend and relationship of the experiment from being affected.