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UE2 Biocell > Techniques d'étude > Flashcards

Techniques d'étude Flashcards

1
Q

Microscope confocale = 3D ?

A

Oui

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2
Q

Resolution ME

A

0.2 nm

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3
Q

Caract MEB

A

Prep echantillon : recouvert de metal

Aspect lisse 3D

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4
Q

Carac MET

A

Coloration negative ac couples bille or => nuance de gris 2D

Cryofracture => relief 3D

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5
Q

Detection cytometrie en flux

A

Fluo et lasers

Charges

Comptage direct ou integre

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6
Q

App ac methodes detection

A

Confo

Cytometrie en flux

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7
Q

Qd confluence

A

Phase finale culture

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8
Q

Piege AC

A

Extra cell

Permeability Necessaire pour intrac

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9
Q

Carac microconfocale

A

Photonique
Fluo
Aspect de tranche pas de relief mais 3D qd même

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10
Q

Utilisation immunofluorescn

A

Confocale

Cytometrie en flux

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11
Q

Culture lignee carac

A

Tuneur

Mois annees

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12
Q

Speci si 2 prots à detecter

A

2 ag et 2 fluorochromesbdiff

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13
Q

Eg fluorochrome

A

DAPI Abs ds UV et emet ds bleu
Fluoroiide abs ds bleu emet ds vert
Rhodospsine abso (=excite) ds vert et emet ds le rouge

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14
Q

AC sscondaire doit reconnaitrw espece 1

A

Non juste ac primaire

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15
Q

Filtre d’arrêt emission pour microscopie confocale

A

Laisser passer 1 seule lambda

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16
Q

Cellule vivante quel micro utiliser

A

Confocale (fluo)

17
Q

Un ac est un ag ?

A

Oui

18
Q

Ac II reconnaît milieu ds lequel AC I creer ?

A

Oui

19
Q

Est ce que ac de la chaine beta reconnait domaine intracellulaire ?

A

Noncar AC NE RECONNAIT JAMAIS domaine IC

20
Q

Synonyme culture en lignee

A

Tumorales

21
Q

Pour visualiser en microscopie confocale, il faut tjs disposer d’ac speci diriges contre la prot d’arret

A

Non pas obli

22
Q

Qui permet 3D micro a fluo

A

Micro confoncal a balayage laser

23
Q

Quelle source de lum micro a flui

A

Photobs

24
Q

Eg intercalant ADN

A

Iodure de propiniun

UE2 Biocell (15 decks)

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