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UE2 Biocell > Mitose > Flashcards

Mitose Flashcards

1
Q

pq MPF ne ressort pas

A

phosp par cycline B et plk inactive suganl exportation

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2
Q

cooperation topoisomerase et ?

A

condensine

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3
Q

struc condensine

A

5 - enserrre 1 boucle ADN de debut prophase a fin anaphase

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4
Q

presence cohesine depuis qd

A

fin de G1

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5
Q

kinetochore cb de prot

A

90 (bus)

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6
Q

centromere composant

A

heterochrom ADN satellite alpha

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7
Q

quelle plaque kinetochore composee de prot passageres

A

interne

arrivee en G2

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8
Q

centromere special ?

A

nucleosomes diff : H3 CENP

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9
Q

prophase centrosome

A

separation et eloignement centrosomes dupliques

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10
Q

prot microtubules prophase

A

dyneide (ancres au cortx et env nucleaire) et kinesin 14

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11
Q

PM fuseau

A

stabilise par facteurs nucleaires => apparition in noyau

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12
Q

reorganisation organites PM

A

Golgi (B2/1 - Plk) => prot => disso des sacs (ben goes to asia)

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13
Q

PM MPF phospho qui

A

lamines AC, pores (avec A1), kinesin 5

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14
Q

origine 3 types de MT

A

des deux centrosomes

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15
Q

comment fueau s’allonge en PM

A

kiensin 5 phos par MPF se dimerise

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16
Q

fuseau vs plan equatorial

A

f : ligne entre les 2 centrosomes

plan equ : ligne des chromoes

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17
Q

separase sequestrre la securine jusquau passage du pt de controle du fuseau

A

non c’est la securine quisequestre la seprase

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18
Q

ct MPF active

A

cdc25

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19
Q

5 etapes cles de la prophase

A
  1. accu de MPF ds le noyau
  2. autoamplification du MPF
  3. condensation chromatine et desassemblage du nucleole
  4. Mise en place du kinetochore
  5. formation de sMT mitotiques
  6. Separation des centrosomes
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20
Q

qd commence le dessasemblage du nucleole

A

G2

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21
Q

qd formation du cx kinetochorecentromere

A

G2

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22
Q

G2 what happens that is linked to prophase

A
  1. desabe nucleole
  2. cx kineto-centromere
  3. arrivee des prot passageres du jkientochore
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23
Q

qd a lieu separation des centrosomes

A

prophase

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24
Q

condensine : monomere ou multimere

A

anneau de pls sous unites

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25
Q

de qd a qd presence de condensine

A

de prophase a fin anaphase

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26
Q

qd arrivee cohesine

A

G1

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27
Q

diff et = cohesine et condensine

A

= : anneau
diff : G1 vs G2
enserre les chromatides soeurs vs enserre boucles ADN vs

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28
Q

ADN du centromere transcrti

A

non

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29
Q

centromere : m place pour 1 paire de chromosome et position tjs id sur fibre chromatid

A

m place pour 1 chromosone

mais places diff sur la fibre chromatidienne

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30
Q

qd arrivent les prot passageres

A

G2

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31
Q

🦁why is centroemre special (2)

A

heterochr non transciere

monomere H3 CENP

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32
Q

pq ADN se condense en prophase 1

A
  1. H1 et H3 hyper efficen -> phosph par MPF et Aurora B
  2. cohesine topoisomerase
  3. consensine
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33
Q

🦁 qui phos h1 et h3

A

MPF et Aurora B

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34
Q

MT sont polarises

A

oui

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35
Q

polymerisation des MT des 2 cotes

A

oui

36
Q

proteines de catastrophe permetten depolyerisation des MT interphasiques

A

oui

37
Q

MT interph vs mitose : taille - mobilite - nb

A

plus petis => plus nbx et plus rapides

38
Q

🦁ct separation des centrosomes

A

dyneide (mais contrbalancee par la kinesin 14 qui fait des ponts entre les 2 MT de pole diff)

39
Q

kinesin 5 ou 14 den prophasr

A

kineisn like 14 (+ vers -)

40
Q

kiensin 14 = prot motrice

A

oui

41
Q

quelle phase formation du fuseau

A

prometaphase

42
Q

pq rupture de l’env nucelaire (3)

A
  1. dyneide => tension sur env
  2. lamines phophs par MPF
  3. Pores nucleaires phosphor par MPF et A1
43
Q

a partir de q phase app de Golgi non fonctionnel

A

prometaphase

44
Q

pq Golgi plus foctionnel

A

1 et B2 phosph prot d’empilement des sacs

45
Q

formation de vesicules qd reorganisation de Golgi

A

oui

46
Q

prometaphase : reorganisation QUE de Golgi

A

non aussi RE

47
Q

q pahse formation du fUSEAU

A

prometa

48
Q

MT kine se fixent a q plaque du kinetochore

A

ext

49
Q

🦁attachement monopolaire des chromosome existent ?

A

oui puis bipolaire

50
Q

MT = constitutant du centrosome

A

oui

51
Q

quelle kinase gere apparienemtn bipolaire des kinetochore

A

Aurora BBBB

52
Q

metaphase : pq de tension des MT kinetochrine –> pq pas separation mainteneant

A

tjs cohesine

53
Q

synonymes plaque metaphsique

A

= ligne mediane = plan equtoril

54
Q

quels num de chromosones ont des constricitn sseocdaire

A

13 14 15 21 22

55
Q

qui gere alignemetndes chromosones

A

condensine

56
Q

cohesine intacte en metaphase

A

non cell des bras phosp par PLK

57
Q

metaphase : Mt immobiles

A

non poly et depol mais L fuseau ctse

58
Q

similarites et differences APC et SCF

A

APC dirige vers le protease mais reconnait les prots non phospho

59
Q

2 conditions du MCP = pt de controle du fuseau

A 
bon alignement (condensine)
app bipolaire (Aurora B)
60
Q

role APC

A

ubiuiqtine cycline A en prometaphase / cycline B et securine en metaphse

61
Q

APc = 1 prot

A

cx proteique

62
Q

pq pas de separation des chromatides avt

A

tjs cohesine -> separase pas activee (phosph par B1) et emprisonnee

63
Q

ct ativation de la separase

A

phosphatase enleve le phosphate de MPF

64
Q

diff anaphase A et B (2)

A

A starts #1 but both happen together
A : chrosmo are pulled polewards (depoly en + mais fuseau cst_
B: poles move apart (allongement du fueau)

65
Q

🦁how migration of poles

A
  1. Mt polaires: ponts g prot motrices=> glissement
  2. MT asteriens plus fixes a MP (deplacemetn d’une dyneide)
    => anaphase B
66
Q

🦁telophase : MT interphasique ou mitotiques

A

interphasque (plus de MPF)

67
Q

ct reconsitituion du noyau et m

A
  • lamines : phosphatases
  • pores : phosphatases
  • no condensine
68
Q

🦁MPF phospoho qui

A
  • seprase
  • myosin light chain
    1. Prophase
    1. Wee1 et cdc25
    2. H1/H3
    3. condensine
      1. Promet
  • lamines
  • pores
  • kinesin 5
69
Q

forme du fuseau : cell filles

A

entonnoire

70
Q

🦁ct sepration des cell

A

anneau ‘'’muscle’’’’ : actine myosine

71
Q

fin division cell : plus de MPF ?

A

non plus de cors iternedia

72
Q

cytodieres ou sont les MT

A

au centre

73
Q

cancer : cycle trop rapide OU trop lent ?

A

oui

74
Q

prot suppreseur de tumeur : cycle cell trop rapide ou trop lent ?

A

trop lent

75
Q

compo chromosome mitotique

A

1/3 adn 1/3 prot non histone 1/3 histones

76
Q

myosine phosphoryle permet contact avec avc actine

A

ys

77
Q

🦁pq pas activation de APC

A

kientochore envoie signal –> prot de controle ne pRT PAS

78
Q

anaphase A vs anaphas eB

A

A : (first) bouger chromosnes (MT kine se depol)

B : bouger les centrosomes –> deplacemnt d’une nydide + prot motricesMT asterine ==> fuseau augmente

79
Q

toposiomeraseutilise pas ATP

A

faux elle utilise

80
Q

comment rupture EN

A

deplacement d’une dyneide –> tension / phos de lamine A/C

81
Q

prophase duplication des centrosomes

A

non en S !

82
Q

ct deplacement des centrosomes en prophase

A

prot motrces : dyeide rte kinesin like 14

83
Q

qui agit sur Golgi

A

Cycline B2 - 1 et PLK

84
Q

ct activation deAPC

A

plk et MPF

85
Q

def plaque metapho

A

attachemnt bipolaire des kinetochores

UE2 Biocell (15 decks)

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