Intro au monde du vivant

Question 1

dimensions eucaryotes

Answer 1

10-30 micrometres

Question 2

nb eucary vs nb pro

Answer 2

10^14 vs 10^15

Question 3

taille C-C, glucose, hemoglobine, bacterie

Answer 3

10^-10,-9,-8,-6

Question 4

Eg procaryotes

Answer 4

Bacteries et archees

Question 5

Leuwanhook a obs les premieres cell animales >

Answer 5

non vegetales

Question 6

ATP : cb de kcal/mol

Answer 6

13

Question 7

reperer mitochondrie dsschem

Answer 7

‘struc en echelle’ double membrane

Question 8

taille du pore nucleaire

Answer 8

100nm

Question 9

taille noyau

Answer 9

microns

Question 10

ct repere les antigenes

Answer 10

fluorochron=mes

Question 11

synonyme MO pas conocal

Answer 11

= MO a fond clair

Question 12

ct insertion mol ds cell

Answer 12

microinjection - electroporation (choc electrique) - transfetion (liposome) = transformation chez un procaryote

Question 13

cell totitpotence

Answer 13

cell souches= peut se diviser en labos

Question 14

but culture cell

Answer 14

multiplier les cell pour les etudier

Question 15

toute MO avec lasers

Answer 15

non juste MO confocal (=> fluo)

Question 16

electroporation permet de faire des pores ds m nucleaire

Answer 16

nnon MP

Question 17

methodes d’intro NECESSITEnt mol fluo

Answer 17

non

Question 18

synonyme micropipette

Answer 18

aiguielle microscop

Question 19

dapi fluo de quel organite

Answer 19

noyau

Question 20

dapi : enzyme

Answer 20

non fluorochrome

Question 21

2 prots diff peuvent etre identifie par 1 meme fluo

Answer 21

non 2 fluorochromes diff (lambda emission et absor diff

Question 22

proteines fluo =fluorohrome

Answer 22

NON

Question 23

besoin d’ac pour mol fluo

Answer 23

NON

Question 24

origines des cell culture oirmaire

Answer 24

tissu oraganes cell

Question 25

origines des cell culture lignees

Answer 25

cell MODIFIEES

Question 26

origines des cell souches

Answer 26

oeuf

Question 27

origines des cell souches

Answer 27

oeuf

Question 28

taile C-C

Answer 28

10-10

Question 29

taille hb

Answer 29

10-8

Question 30

15% de bruit de fond a prendre en compte

Answer 30

NON on ne prend JAMAIS en compte bruit de fond

Question 31

ac : absence de prot ds schema donc absence de prot

Answer 31

non prot peut etre a l’int car ac = que surface

Question 32

diff ac directe ou indirect

Answer 32

direct: esp peu etre id - indirect : TJS esp diff

Question 33

ag que prot

Answer 33

non virus bacterie cell mole

Question 34

ac ou ag = prot

Answer 34

ac

Question 35

anticorps primaire B = ag de anticorps secondaire C

Answer 35

oui

Question 36

ou fluorochorme ds imuuno indirecte

Answer 36

Ac-1 - AC2 - fluo

Question 37

hematoxyline et eosine = maruquers vitaux ?

Answer 37

oui

Question 38

immunocytochimie repose sur emploi de prot fluo

Answer 38

oui

Question 39

immunocytochimie et dc immunocfluo repose sur 3 quoi

Answer 39

1. maruquerus vitaux
2. ac specifiques diriges contre ele cell
3. prot fluo