Intro au monde du vivant Flashcards

1
Q

dimensions eucaryotes

A

10-30 micrometres

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2
Q

nb eucary vs nb pro

A

10^14 vs 10^15

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3
Q

taille C-C, glucose, hemoglobine, bacterie

A

10^-10,-9,-8,-6

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4
Q

Eg procaryotes

A

Bacteries et archees

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5
Q

Leuwanhook a obs les premieres cell animales >

A

non vegetales

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6
Q

ATP : cb de kcal/mol

A

13

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7
Q

reperer mitochondrie dsschem

A

‘struc en echelle’ double membrane

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8
Q

taille du pore nucleaire

A

100nm

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9
Q

taille noyau

A

microns

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10
Q

ct repere les antigenes

A

fluorochron=mes

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11
Q

synonyme MO pas conocal

A

= MO a fond clair

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12
Q

ct insertion mol ds cell

A

microinjection - electroporation (choc electrique) - transfetion (liposome) = transformation chez un procaryote

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13
Q

cell totitpotence

A

cell souches= peut se diviser en labos

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14
Q

but culture cell

A

multiplier les cell pour les etudier

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15
Q

toute MO avec lasers

A

non juste MO confocal (=> fluo)

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16
Q

electroporation permet de faire des pores ds m nucleaire

17
Q

methodes d’intro NECESSITEnt mol fluo

18
Q

synonyme micropipette

A

aiguielle microscop

19
Q

dapi fluo de quel organite

20
Q

dapi : enzyme

A

non fluorochrome

21
Q

2 prots diff peuvent etre identifie par 1 meme fluo

A

non 2 fluorochromes diff (lambda emission et absor diff

22
Q

proteines fluo =fluorohrome

23
Q

besoin d’ac pour mol fluo

24
Q

origines des cell culture oirmaire

A

tissu oraganes cell

25
origines des cell culture lignees
cell MODIFIEES
26
origines des cell souches
oeuf
27
origines des cell souches
oeuf
28
taile C-C
10-10
29
taille hb
10-8
30
15% de bruit de fond a prendre en compte
NON on ne prend JAMAIS en compte bruit de fond
31
ac : absence de prot ds schema donc absence de prot
non prot peut etre a l'int car ac = que surface
32
diff ac directe ou indirect
direct: esp peu etre id - indirect : TJS esp diff
33
ag que prot
non virus bacterie cell mole
34
ac ou ag = prot
ac
35
anticorps primaire B = ag de anticorps secondaire C
oui
36
ou fluorochorme ds imuuno indirecte
Ac-1 - AC2 - fluo
37
hematoxyline et eosine = maruquers vitaux ?
oui
38
immunocytochimie repose sur emploi de prot fluo
oui
39
immunocytochimie et dc immunocfluo repose sur 3 quoi
1. maruquerus vitaux 2. ac specifiques diriges contre ele cell 3. prot fluo