TCA Cycle and ETC Flashcards

1
Q

What is the function of the pyruvate dehydrogenase complex?

A

Converts pyruvate to acetyl CoA at the end of glycolysis

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2
Q

What are the most important products of the TCA cycle for ATP production?

A

reduced coenzymes

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3
Q

How many ‘steps’ are there in the PDC?

A

Three

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4
Q

Name a cofactor that is used by PDC and an example of it’s source

A

thiamine pyrophosphate (TPP) from vitamin B1

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5
Q

How many complexes is the ETC comprised of?

A

Four

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6
Q

Describe what happens at complex I

A

NADH converted to ubiquinone

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7
Q

Describe what happens at complex II

A

FADH converted to ubiquinone

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8
Q

Describe what happens at complex III

A

Ubiquinone converted to cytochrome C

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9
Q

Describe what happens at complex IV

A

Cytochrome C to oxygen

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10
Q

Give a reason as to why ATP wouldn’t be produced from the ETC

A

If there charge (electrochemical/proton gradient) is neutralised or if there is another way for protons to pass across the membrane other than through ATP synthase

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11
Q

How do ‘natural antibiotics’ work?

A

They affect coupling reactions (alter the movement of protons across the membrane to release ATP) in bacteria.

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12
Q

How is PDC regulated?

A

Feedback inhibition and cavalent modification through phosphorylation y both PDH kinase and protein kinase A

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13
Q

How is the TCA cycle controlled?

A

Rate depends upon NAD+ availability so is essentially substrate controlled as well as linked to ATP:ADP ratio as NAD+ availability is dependent on rate of ETC

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14
Q

What metabolic adaptations occur in hypoxia?

A

Limit ATP use by switching off non-essential cell functions, improve anaerobic ATP production efficiency and limit oxidative stress by preventing entry to mitchondria (preventing TCA and ETC)

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15
Q

How are metabolic adaptations to hypoxia mediated via HIF1?

A

Can be mediated via changes to gene expression. In normoxia HIF1a subunit degraded where sin hypoxia it is stabilised and binds to upstream elements of promoters and causes the down-regulation of mitcohondrial respiration as well as promoting loss of mitochondria (to prevent risk of oxidative stress and ischaemia)

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16
Q

What is meant by ‘HIF1’?

A

hypoxia inducible factor 1 (a transcription factor)