T7 ETC and Oxidative Phosphorylation

Question 1

Outline the steps involved in oxidative phosphorylation

Answer 1

NADH and FADH2 come from Krebs cycle and ‘drop off’ Hs at ETC

Hs split into protons and electrons

Electrons go along the ETC being put into lower energy levels

This provides energy which powers the proton pump - protons are actively transported into the Intermembrane space forming the proton pool

This forms an electrochemical gradient

H+ travel by chemiosmosis from proton pool down stalked particle

Then travel through ATP synthase which produces energy for phosphorylation of ADP to ATP

The end result is e- from ETC and H+ from chemiosmosis in the matrix

These combine with oxygen breathed in to produce water

Question 2

Equation for the formation of water in oxidative phosphorylation

Answer 2

2H+ + 2e- + 1/2O2 > H2O