stuff you need to know but don't lol

Question 1

how can GFP be used to check redox state?

Answer 1

insert two Cys residues into GFP–>the colour and intensity of the GFP depends on the redox state of the two Cys residues

Question 2

what reports of glutathione redox potential?

Answer 2

roGFP2–preferentially interacts with glutaredoxins