Kinetics

Question 1

MM eq’n

Answer 1

V0= Vmax[S]/(Km+[S])

Question 2

what defines an enzyme’s catalytic ability?

Answer 2

Km and Vmax

Question 3

Km= (k2+k-1)/k1

Answer 3

ya

Question 4

when k2 is &laquo_space;than k-1, Km= k-1/k1= Kd

Answer 4

ya

Question 5

how can you tell what kind of complex is formed by looking at a Lw-B plot?

Answer 5

if a ternary complex is formed, there will be an intersection of all the lines; if a tern. complex is not formed, all lines will be parallel

Question 6

what is rasagiline?

Answer 6

Rasagiline is a suicide inhibitor–important in parkinson’s disease

• -inhibits monamine oxidases
• -dopamine neurons get destoryed, dopamine decrease–monamine oxidases digest dopamine, so if you inhibit MAO you have higher levels of dopamine

Question 7

what is the scatchord plot eq’n?

Answer 7

[LR]/[L]=[Rt]/Kd-[LR]/Kd

Question 8

eq’ns for Kd

Answer 8

k-1/k1=[R][L]/[LR]= 1/Kassociation

Question 9

free energy in terms of Ka

Answer 9

dG= -RT (lnKa)

Question 10

LR eq’n

Answer 10

[Rt][L]/[Kd+[L]]

Question 11

when [S] is &laquo_space;Km then:

Answer 11

Vo=(kcat/Km) * [Et][S]

Question 12

kcat/Km is a second order rate constant–>uper limit is 10e8 M-1 s-1

Answer 12

ya–with an enzyme that has a value close to this is called “diffusion controlled” and has reached catalytic perfection

Question 13

what is the Arrhenius eq’n?

Answer 13

kcat= Ae^(-Ea/RT)