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Microbiology Lab Practicum #2 > Strep Tests > Flashcards

Strep Tests Flashcards

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1
Q

What are the six strep tests?

A
  1. bile esculin test 2. BHI + 6.5% NaCL test 3. CAMP test

4. Bacitracin susceptibility test 5. SXT susceptibility. test 6. Hemolysis

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2
Q

What is the purpose of the bile esculin test?

A

to determine if your organism can hydrolyze esculin in the presence of bile

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3
Q

What is the application of the bile esculin test?

A

identify enterococci and other group d strep

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4
Q

What media is used for the bile esculin test?

A

bile esculin agar

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5
Q

What is found in bile esculin agar?

A

peptone (nutrients), bile (inhibit gram + bacteria except enterococci and group d strep), ferric citrate (color indicator), esculin (substrate)

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6
Q

What is the inoculation guide and incubation for the bile esuclin test?

A

loopful streak on slant; standard

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7
Q

What is the theory of the bile esculin test?

A

esculin turns into glucose/ esculetin, ferric citrate –> dark brown precipitate

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8
Q

What are the results of the bile esuclin test?

A

(+) dark brown (-) no change

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9
Q

What is the purpose of the BHI 6.5% NaCL test?

A

to determine if an organism will grow on BHI + 6.5% Nacl

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10
Q

What is the application of the BHI 6.5% NaCL test?

A

ID enterococci (+) VS group d strep (-)

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11
Q

What is the media used for BHI 6.5% NaCL test?

A

brain heart infusion + 6.5% Nacl broth

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12
Q

What is the inoculation and incubation for the BHI 6.5% NaCL test?

A

loopful, standard

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13
Q

What are the results of the BHI 6.5% NaCL test?

A

(+) growth

(-) no growth

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14
Q

What is the purpose/ application of the CAMP test?

A

identify Streptococcus agalaciaea

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15
Q

What media is used for the CAMP test?

A

blood agar

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16
Q

What is the inoculation guide for the CAMP test?

A

Streak Staphylococcus CAMP at the bottom of the blood agar, streak a lawn on upper half of BAP with your organism, make two streaks of your organism perpendicular to SAC.

17
Q

What is the incubation of the CAMP test?

A

standard

18
Q

What is the theory of the camp test?

A

synergistic affects of staphylococcus aureus camp and streptococcus agal. produce a unique arrow pattern

19
Q

What are the results of the camp test?

A

(+) arrow pattern

(-) no arrow pattern

20
Q

What ist he BAC application?

A

ID BAC sensitive Strepto. pyogenes

21
Q

What are the BAC results?

A

S: ZOI > 10 mm
R: ZOI < `10 mm

22
Q

What are the SXT results?

A

S: ZOI present
R: ZOI not present

23
Q

What other three tests can be used during the CAMP test?

A

Hemolytic patterns, BAC and SXT

Microbiology Lab Practicum #2 (16 decks)

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