Exoenzymes

Question 1

What are enzymes?

Answer 1

proteins that catalyze biological reactions

Question 2

Where do endoenzymes function?

Answer 2

inside of the cell

Question 3

What are exoenzymes?

Answer 3

Enzymes that are released from the cell to catalyze reactions outside of the cell

Question 4

In the casein hydrolysis test, what exoenzyme is being tested?

Answer 4

caseinase

Question 5

What media is used for the casein hydrolysis test?

Answer 5

skin milk agar

Question 6

What is the inoculation and incubation guide for the casein hydrolysis test?

Answer 6

loopful streak, 37C for 48 hours

Question 7

What is the theory of the casein hydrolysis test?

Answer 7

caseinase turns casein into amino acids

Question 8

What are the results for the casein hydrolysis test?

Answer 8

+- clear around growth - : white around growth

Question 9

Which exoenzyme is being tested in the starch hydrolysis test?

Answer 9

amylase

Question 10

What media is used for the starch hydrolysis test?

Answer 10

starch agar plate

Question 11

What is the inocoluation guide for the starch hydrolysis test?

Answer 11

lapful streak

Question 12

What is the incubation period for the starch hydrolysis test?

Answer 12

37C for 48 hours, after incubation add iodine all over plate

Question 13

What is the theory for the starch hydrolysis test?

Answer 13

amylase turns starch into simple sugars

Question 14

What is the result for the starch hydrolysis test?

Answer 14

(+)- yellow halo around growth (-)- blue/black around growth

Question 15

Which media is this?

Answer 15

Starch agar plate

Question 16

Which media is this?

Answer 16

Skim milk agar