Gelatin Hydrolysis test/ Urea Hydrolysis Test

Question 1

What is the purpose of the gelatin hydrolysis test?

Answer 1

to detect the presence of gelatinase

Question 2

What media is used for the gelatin hydrolysis test?

Answer 2

Tryptic soy broth (TSB)

Question 3

What is the inoculation guide for the gelatin hydrolysis test?

Answer 3

loopful inoculation. after first inoculation add a gelatin strip and then reincubate

Question 4

What is the incubation period for the gelatin hydrolysis test?

Answer 4

37C for 48 hours

Question 5

What is the theory for the gelatin hydrolysis test?

Answer 5

gelatinase will break down gelatin into amino acids

Question 6

What are the results of the gelatin hydrolysis test?

Answer 6

(+)- clear blue strip

-)- green/ gray strip (no change

Question 7

What is the purpose of the urea hydrolysis test?

Answer 7

detect the presence of urease

Question 8

What is the application for the urea hydrolysis test?

Answer 8

help differentiate GNR

Question 9

What media is used for the urea hydrolysis test? What else is found in this media?

Answer 9

TSA plate with previously grown culture
-1 mL of water
- urea tablet
   Yeast abstract: nutrients
   Urea: substrate for urease
   Phenol red: pH indicator

Question 10

What is the inoculation and incubation guide for the urea hydrolysis test?

Answer 10

heavy inoculum into 1 mL of water, add urea tablet, incubation 37C for 48 hours

Question 11

What is the theory behind the urea hydrolysis test?

Answer 11

urease breaks down urea into 2NH3 (ammonia and CO2)

Question 12

What are the results of the urea hydrolysis test?

Answer 12

(+)- shocking pink

(-)- yellow, peach