IMViC Tests

Question 1

What are the IMViC tests?

Answer 1

A series of 4 tests to differentiate Enterobacteriaceae (Gram (-) rod)

Question 2

What are the names of the four tests of the IMViC tests?

Answer 2

I= indole production test M= methyl red test V= voges proscaur test C= citrate utilization test

Question 3

What is the purpose of the indole production test?

Answer 3

determine if an organism has the enzyme tryptophanase

Question 4

What media is used in the indole production test?

Answer 4

tryptone broth

Question 5

What is the inoculation and incubation guide for the indole production test? What do you do after incubation?

Answer 5

loopful, 37C for 48 Hours, add Kovac’s reagent and indole (yellow) rosindole= pink

Question 6

What are the results of the indole production test?

Answer 6

+=pink - = yellow

Question 7

What is the purpose of the MRVP test?

Answer 7

to determine if your organism ferments glucose into mixed acids (MR test) or a neutral product called acetoin (VP test)

Question 8

What media is used for the MRVP tests?

Answer 8

glucose supplemented nutrient broth

Question 9

What is the inoculation and incubation guide for the MRVP test?

Answer 9

loopful, 37C for 48 hours

Question 10

What is the theory for the indole production test?

Answer 10

tryptophanase turns tryptophan into pyruvic acid, NH3 (ammonia) and indole

Question 11

What is the theory of the MR test?

Answer 11

glucose is fermented to mixed acid

Question 12

What do you do after incubation during the MR test?

Answer 12

add methyl red (pH indicator) Acidic: red, neutral/basic: yellow

Question 13

What are the results of the MR test?

Answer 13

(+)- red (-) - yellow

Question 14

What is the theory for the VP test?

Answer 14

glucose is fermented into a neutral product

Question 15

What do you add after incubation for the VP test to detect acetoin?

Answer 15

alpha-naphthol and KOH (potassium hydroxide)

Question 16

What are the results of the VP test?

Answer 16

(+)-red (-)- yellow or color of media

Question 17

What is the purpose of the citrate utilization test?

Answer 17

to determine if your organism can use sodium-citrate as the only carbon source

Question 18

What media is used for the citrate utilization test? What are the three parts of it?

Answer 18

simmons citrate agar slant -sodium citrate is the only carbon source -ammonium phosphate is the only nitrate source - Bromthymol Blue (pH indicator) -acidic: yellow - neutral: green -basic: blue

Question 19

What is the inoculation guide for the citrate utilization test?

Answer 19

1 loopful streak on slant

Question 20

What is the incubation period for the citrate utilization test?

Answer 20

37C for 48 hours

Question 21

What is the theory for the citrate utilization test?

Answer 21

if the organism can use ammonium phosphate and NA citrate as only C and N course then ammonia is produced (NH3)

Question 22

What are the results of the citrate utilization test?

Answer 22

(+) growth, blue (-) no growth, green

Question 23

Which is positive for being able to use sodium-citrate as the only carbon source?

Answer 23

Blue

Question 24

Which is postive for pyruvic acid, NH3, and indole?

Answer 24

The one with the pink line at the top