Staphylococcus and micrococcus - Flashcards
Staphylococcus and micrococcus - Morphology
coccus 1um in membranes, soil, water, food no spore no flagella no capsule
Staphylococcus and micrococcus - staining and culture
Gram positive
Simple,
micrococcus has yellow pink orange colonies,
staphylococcus has white colonies
S. aureus ssp. anaerobius has no pigment
Selective culture on mannitol 10Nacl black collonies
Staphylococcus and micrococcus - Fastidious or air tolerance
Non fastidious
Staphylococcus is facultative anaerobic apart from S. aureus ssp. anaerobius.
Micrococcus is obligate aerobic
Staphylococcus and micrococcus - Catalase, oxidase and OF, haemolysis
Catalase +
Oxidase -
OF - F
alpha or beta haemolysis
Staphylococcus and micrococcus - species
S. aureus ssp aureus, S aureus ssp. anaerobius, S. pseudointermedius S. hyicus S. Gallinarium S. felis S. equorum
Staphylococcus and micrococcus - Virulence factors
Coagulase Protein A Lipase esterase elastase hyaluronase staphylo kinase leukocidin - destruction of phagocytes alpha toxin - muscle spasm (gangreen) beta toxin
Staphylococcus and micrococcus - Micrococcus
Saprophyte obligate aerobic Catalase + Oxidase + OF O/-