Mycobacterium

Question 1

Mycobacterium - Morphology and Environment

Answer 1

soil and water,
obligate pathogen in host and facultative in environment
rods, coccoid,
Cell wall has mycolicacids, lipids and waxs
No flagella wall or capsule

Question 2

Mycobacterium - Staining and culture

Answer 2

Gram positive,
ziehl-neelson positive acid/alcohol fast
cell wall cant be stained so use fluorescent dyes like methyl blue
Broth; henley sauton chord
Solid; egg, serum agar or glycerine potatoe
Selective; petragnani(green), acid treatment or animal trial.

Question 3

Mycobacterium - Biochemistry and antigens

Answer 3

Catalase +
Oxidase -
Glycolipids and lipoproteins
shared antigens - cross reactions and immuno electrophoresis
Tuberculin - Broth culture bioled and purified to PPD

Question 4

Mycobacterium - Obligate Pathogens

Answer 4

M. tuberculosis - hu, su, dog, cat, parrot, cattle
M. africanum - Hu
M. bovis - Ru, Su, Car, Hu, BCG
M. Caprae - Ru, Su,Hu
M. microti - rodents
M. avium ssp. avium - birds
M. avium ssp. paratuberculosis - Ru paratuberculosis, johnes disease incubation for two days,
M. avium ssp. silvaticum - birds TB but cattle PTB
M. leprae - Hu leprosy
M. lepraemurium - mouse and cats leprosy

Question 5

Mycobacterium - Facultative

Answer 5

M. fortinium
M. marinium
su and ru local lesions

Question 6

Mycobacterium - Groups

Answer 6

photochromogen
scotochromogens
non-chromogens
rapid growers

Question 7

Mycobacterium - Two species you cannot culture

Answer 7

M. leprae

M. lepraemurium