Bacillus

Question 1

Bacillus morphology

Answer 1

4-5um rod, central spore, capsule, no flagella on anthracus but there is on the others.

Question 2

Staining bacillus

Answer 2

Gram positive, toulidine blue gives a pink membrane.

Question 3

pathogenicity bacillus

Answer 3

Obligate pathogen, capsule has D-glu polypeptides, polysaccharide in hapten wall, cross reaction with B. cerus

Question 4

Culture

Answer 4

Simple
liquid is cotton looking
rough and smooth, smooth in 10-15% CO2 capsule.

Question 5

Spore in a carcass?

Answer 5

No it needs - 15deg, water and O2

Question 6

Metabolism

Answer 6

Active, catalase + oxidase -

Question 7

Virulent factors of B. anthracus

Answer 7

Plasmid coded - Odema and lethal factor, protective antigen

Question 8

How resistant is the spore?

Answer 8

decades in environment, 2 weeks in skin, 4 weeks in bone

Question 9

species of Bacillus

Answer 9

Anthracis, cerus, lichiformus, paenibacillus larvae, subtilis, megaterium, thuringiensis.

Question 10

Species affected by B. anthracis

Answer 10

Mainly herbivores, car quite resistant, birds completely resistant.
Eq; subacute, oedema, colic, enteritis and septicaemia.
Su; oedema esp in pharyngeal area, intestinal more lethal form is less common.
Cattle and sheep; peractute septecaemic anthrax
Hu; skin pulmonary and intestinal form.

Question 11

B. cerus

Answer 11

mastitis in cow, eye infection in Hu

Question 12

B. lichiformus

Answer 12

abortion in cow and sheep

Question 13

B. thuringiensis

Answer 13

pest control