Erysipelothrix, Listeria -

Question 1

Erysipelothrix, Listeria - Morphology

Answer 1

Widespread, 50% of pigs tonsular tissue, wild birds and animals
1-3um thin curved rods
thin capsule
no flagella
no spore

Question 2

Erysipelothrix, Listeria - Staining and culture

Answer 2

SR colonies - 
acute=S,uniform terbidity 
Chronic=R, sediment
Blood agar alpha haemolysis after 48hrs 
pin colonies
selective NaN3 and crystal violet or neomycin

Question 3

Erysipelothrix, Listeria - Virulence factors and antigens

Answer 3

Coagulase
hyaluronase
neaurmidase
Antigens two common protective and heat stable cell wall polysaccharide haptens 1-26 1a 1b and 2 are common

Question 4

Erysipelothrix, Listeria - Biochemistry

Answer 4

Catalase -
Oxidase -
Fermentative 
H2S production
facultative pathogenic

Question 5

Erysipelothrix, Listeria - Species of Erysipelothrix

Answer 5

E. Rhusiopathiae - diamond skin disease, erysiupelis on su, valvular endocarditis, arthritis,
sheep - post dipping lameness, valvular endocarditis poly arthritis in lambs
Bird - endocarditis
Hu - erysepeloid
E.Tonsillarium - differeentialed from E.r one of several serotypes,not pathogenic for swine, in tonsils
canine endocarditis.

Question 6

Listeria - Morphology and Environment

Answer 6

wide distribution
can replicate in environment
1-2um short thick rods,
flagella at room temperature - tumbling motility

Question 7

Listeria - Staining and culture

Answer 7

Simple
Facultative anaerobic
alpha or beta haemolysis
4-45deg PH5.5-9.5
Selective - cattle serum, trypaflavin, nalidixix acid
cold enrichment

Question 8

Listeria - Biochemistry

Answer 8

catalase +
Oxidase - 
fermentative
13 serotypes 1/2a 4b
cell wall polysaccharide
flagellar protein

Question 9

Listeria - Pathogenicity and virulence factors

Answer 9

Facultative pathogen
haemolysis, 
liposlysis, 
monocytosis factor
cell wall lipoids
listeriosis

Question 10

Listeria - species

Answer 10

L. monocytogens - Ru, encephalitis ‘circling disease’ and abortion in silage fed animals late in pregnancy, sept’
dog cat pig hu eq and birds - septacaemia abortion and encephalitis
L. ivanovii - ov,bo abortion
L. innocua - ov meningoencephalitis