Staph

Question 1

Nutritionally variant staph

Answer 1

Media must have nutrients (heme, thiamin)

Question 2

Staph food poisoning

Answer 2

ENTEROtoxin A and D

no fever, no neurological problems

Question 3

Scalded Skin Syndrome/Ritter’s Disease

Answer 3

Exfoliatin toxin,
erythema followed by skin peeling (face, neck, axilla)
2-4 until spontaneous recovery

Question 4

Toxic Shock Syndrome

Answer 4

rash, fever, hypotension, DIC , shock

TSST1

Question 5

Staph aureus pathogenicty

Answer 5

Enterotoxins: heat stable; nausea/vomit; 5 Types (A-E, H, I) (F = TSST1)

Exfoliatin Toxin: Dissolves mucopolysaccharide matrix of epidermidis and cleaves granulosom layer

Encapsulation: Produces polysaccaharide capsule that inhibits phaygocytosis

Coagulase: Inhibits phaygocytosis through deposition of fibrin on bacterial cell wall.

Hyaluronidase: cleaves hyaluronic acid, spreading factor

Lipase: Hydrolyzes lipid, allows colonization of sebaceous gland

Protein A: Cell wall component, binds to FC of IgG, inhibits phaygocytosis

Question 6

Slide Coagulase

Answer 6

“Clumping Factor”, BOUND coagulase

Question 7

Tube Coagulase

Answer 7

FREE coagulase

Question 8

Oxacillin

Answer 8

Used to determine methicillin resistance