molecular

Question 1

Room A

Answer 1

reagent preparation for PR components

This is a clean room where to nucleic acid is allowed

Question 2

Room B

Answer 2

Nucleic Acid extraction room with hoods

Question 3

Room C

Answer 3

Thermal cycling room for specimen amplificaion

Question 4

Room D

Answer 4

Agarose gel electrophoresis room

Question 5

PCR

Answer 5

target amplification using DNA polymerase and thermo cycling

Question 6

TMA

Answer 6

target amplification using reverse transcriptase. NO thermal cycling needed,

Question 7

bDNA

Answer 7

signal amplification using alkaline phosphatase and NO thermal cycling

Question 8

NASBA

Answer 8

target amplification using 3 enzymes and NO thermal cycling

Question 9

Missense

Answer 9

a nucleotide or sequence substitution that codes for a different amino acid

Question 10

SNP

Answer 10

Most common; a single base change

Question 11

Deletion

Answer 11

A missing nucleotide or other portion of DNA sequence

Question 12

Insertion

Answer 12

An extra DNA nucleotide or other portion of DNA sequence

Question 13

Nonsense

Answer 13

Nucleotide substitution that ends in early termination of the protein manufacturing process; usually due to a stop codon