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Micro > Stains and Cultures > Flashcards

Stains and Cultures Flashcards

1
Q

Microbes with gram stain limitations

A 
These Microbes May Lack Real Color:
Treponema
Mycobacteria
Mycoplasma
Legionella 
Rickettsia
Chlamydia
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2
Q

Giemsa stain

A 
Chlamydia
Borrelia
Rickettsiae
Trypanosomes
Plasmodium
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3
Q

PAS

A

Stains glycogen, mucopolysaccharides

Used to diagnose Whipple’s disease

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4
Q

Ziehl-Neelsen (carbol fuchsin)

A

Acid-fast organisms (Nocardia, Mycobacterium)

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5
Q

India ink

A

Cryptococcus neoformans (can also use mucicarmine)

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6
Q

Silver stain

A

Fungi - pneumocystis
Legionella
H Pylori

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7
Q

Special culture for H Flu

A

Chocolate agar with factors V (NAD+) and X (hematin)

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8
Q

Special culture for N. gonorrhoeae, N. meningitidis

A

Thayer-Martin (or VPN) media

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9
Q

How does Thayer-Martin media work

A

VPN:
Vanc - inhibits gram positive
Polymyxin - inhibits gram negatives except neisseria
Nystatin - inhibits fungi

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10
Q

Special culture for B. pertussis

A

Bordet-Gengou (potato) agar

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11
Q

Special culture for C. diphtheriae

A

Tellurite agar, Loffler medium

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12
Q

Special culture for TB

A

Lowenstein-Jensen agar

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13
Q

Special culture for M. pneumoniae

A

Eaton agar - requires cholesterol

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14
Q

Special culture for lactose-fermenting enterics

A

Pink colonies on MacConkey agar

E Coli also grown on eosin-methylene blue (EMB) agar as colonies with green metallic sheen

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15
Q

Special culture for legionella

A

Charcoal yeast extract agar buffered w/cysteine and iron

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16
Q

Special culture for Fungi

A

Sabouraud agar

Micro (8 decks)

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