Stains Flashcards

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1
Q

What are the 5 types of Stains?

A
  1. Giemsa
  2. PAS (Periodic acid-Schiff)
  3. Ziehl-Neelsen (carbol fuchsin)
  4. India ink
  5. Silver stain
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2
Q

How would you observe Treponemes?

A
  1. Dark-field microscopy

2. Fluorescent antibody staining

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3
Q

How would you observe Legionella?

A

Silver stain

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4
Q

Bugs stained with Giemsa?

A
Certain Bugs Really Try my Patience
Chlamydia
Borrelia
Rickettsiae
Trypanosomes
Plasmodium
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5
Q

Bugs stained with PAS (Periodic acid-Schiff)?

A

Tropheryma whipplei for diagnosing Whipple dz

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6
Q

What does PAS stain?

A

Glycogen, mucopolysaccharides

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7
Q

Bugs stained with Ziehl-Neelsen (carbol fuchsin)

A

Acid-fast organisms

  1. Nocardia
  2. Mycobacterium
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8
Q

Bug stained with India ink

A

Cryptococcus neoformans

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9
Q

To stain Cryptococcus neoformans, other than india ink, what stain can be used?

A

Mucicarmine can also be used to stain thick polysaccharide capsule red

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10
Q

Bugs stained with silver stain

A
  1. Fungi (Pneumocystis)
  2. Legionella
  3. Helicobacter pylori
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