Bacterial genetics Flashcards
1
Q
what are the 4 types of bacterial genetics?
A
- transformation
- conjugation
- transposition
- transduction
2
Q
what is transformation?
A
ability to take up naked DNA from environment
3
Q
what bacterias are able to do transformation?
A
SHiN
S. pneumoniae
H. influenzae type B
Neisseria
4
Q
how can you prevent transformation?
A
by adding deoxyribonuclease to environment will degrade naked DNA in medium –> no transformation seen.
5
Q
what are the 2 types of conjugation?
A
- F+ X F-
2. Hfr X F-
6
Q
What does F+ plasmid contain?
A
F+ plasmid contains genes required for sex pilus and conjugation.
7
Q
what is high-frequency recombination (Hfr) cell?
A
F+ plasmid can become incorporated into bacterial chromosomal DNA then that cell becomes Hfr cell.