Special culture requirements

Question 1

Media for H. influenzae?

Answer 1

Chocolate agar with factors V (NAD+) and X (hematin)

Question 2

Media for N. gonorrhoeae, N. meningitidis

Answer 2

Thayer-Martin (or VPN) media
Vancomycin: inhibits gram +
Polymyxin: inhibits gram - except Neisseria
Nystatin: inhibits fungi

Question 3

Media for B. pertussis

Answer 3

Bordet-Gengou (potato) agar (Bordet for Bordetella)

Question 4

Media for C. diphtheriae

Answer 4

1. Tellurite agar

2. Loffler medium

Question 5

Media for M. tuberculosis

Answer 5

Lowenstein-Jensen

Question 6

Media for M. pneumoniae

Answer 6

Eaton agar, requires cholesterol

Question 7

Media for Lactose-fermenting enterics

Answer 7

Pink colonies on MacConkey agar (fermentation produces acids –> turning the colongy pink)

Question 8

Specific media for E. coli?

Answer 8

Eosin-Methylene blue (EMB) agar as coloniges with green metallic sheen

Question 9

Media for Legionella?

Answer 9

Charcoal yeast extract agar buffered with cysteine and iron

Question 10

Media for Fungi?

Answer 10

Sabouraud agar (Sab’s a fun guy!)