Gram stain limitations Flashcards
1
Q
What are the bugs that do not Gram stain well?
A
These Microbes May Lack Real Color. Treponema Mycobacteria Mycoplasma Legionella pneumophila Rickettsia Chlamydia
2
Q
what is the reason that treponema is not well stained by gram?
A
too thin to be visualized
3
Q
what is the reason that mycobacteria is not well stained by gram?
A
high lipid content
4
Q
how do you stain Mycobacteria?
A
high lipid content in cell wall is detected by carbolfuchsin in acid-fast stain
5
Q
what is the reason that mycoplasma is not well stained by gram?
A
b/c there is no cell wall
6
Q
what is the reason that Leigonella is not well stained by gram?
A
primarily intracellular
7
Q
what is the reason that Rickettsia is not well stained by gram?
A
intracellular parasite
8
Q
what is the reason that Chlamydia is not well stained by gram?
A
- intracellular parasite
2. lacks muramic acid in cell wall
