Staining Procesures

Question 0

What is morphology of E.Coli

Answer 0

Rod shaped

Question 1

How do you make a smear?

Answer 1

1Label clean slide
2Flame loop and obtain 1 loop ful of water
3 flame loop cool flame top of test tube and obtain small amount of bacteria
4. Add bacteria to water
5. Reflame loop and make sure tube reflamed and lid put on
6. Air dry
7. Heat fix

Question 2

What is morphology of Bacillus megatherium

Answer 2

Long rod shape

Question 3

What is morphology of mycobacterium?

Answer 3

Small rods

Question 4

What is the morphology of staphylococcus?

Answer 4

Grape like

Question 5

How do you make gram stain?

Answer 5

1. Smear
2. Crystal Violet–> 1 minute
3. Rinse with water
4. Iodine –> 1 min.
5. Decolonize with Ethyl alcohol
6. Rinse with water
7. Add safes in —> 1 minute
8. Blot dry –> view on oil immersion

Question 6

Gram positive is what color?

Answer 6

Purple

Question 7

Gram negative is what color

Answer 7

Reddish color

Question 8

How do you do an acid-fast stain?

Answer 8

1. smear
2. Add carbon fuchsin –> microwave 1 minute
3. Cool, decolonize with acid alcohol –> 15 seconds
4. Rinse with water
5. Add methylene blue–> 1 min
6. Rinse with water
7. Blot dry –> view on oil immersion lens

Question 9

What color is acid fast positive?

Answer 9

Bright pink

Question 10

What color is acid fast negative?

Answer 10

Blue

Question 11

How do you make a spore stain?

Answer 11

1. smear
2. Add malachite green –> microwave for 1 minute
3. Cool and rinse with water
4. Add safaris fir 45 Sec
5. Rinse with water
6. Blot dry view on oil immersion

Question 12

What color is spore positive?

Answer 12

Green and red
Green(indicate spores)
Red is cell wall

Question 13

What color is sore stain negative?

Answer 13

Red