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Microbiology > chapter 3 > Flashcards

chapter 3 Flashcards

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Biology 101 flashcards
1
Q

what are the units of microscopes in order?

A

mm –> um –> nm goes over three decimal places to left each

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2
Q

what are uses for compound light microscopes

A

stained or live specimens and count microbes

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3
Q

what ocular lens do?

A

to view it is 10x magnification

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4
Q

what does illuminator do?

A

light source

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5
Q

what is the condenser do?

A

focuses light

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6
Q

what are the objective lens?

A 
to magnify
4x
10x
40x
100x
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7
Q

what is the total magnification?

A

objective x ocular

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8
Q

what is the features of phase contrast microscope?

A

special condenser that improves contrast

between specimen and surrounding medium

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9
Q

what are the uses of phase contrast microscopes?

A

live, unstained specimens; internal structure

motility, size, and shape

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10
Q

what are the features of darkfield microscopes?

A

uses darkfield condenser

specimen appears light against black backgroudn

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11
Q

what are the uses of darkfield microscopes

A

living microbes that are invisible in bightfield

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12
Q

what are the features of fluorescence microscope?

A

UV light to illuminate fluorescent microbes

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13
Q

what are the uses of fluorescence microscope?

A

Antibody research to identify microbes

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14
Q

what are the uses of electron microscope?

A

uses beam of electrons focused by electromagnetic lenses
specimen are pretreated with chemical or dyes
specimen are dead

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15
Q

what are the uses of electron microscope?

A

detained internal and external structures

viewing viruses

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16
Q

what are the types of electron microscopes?

A

scanning (SEM)

transmission (TEM)

17
Q

what doe SEM feature?

A

detailed 3D images
whole cells are coated with gold, an electron beam passes over cells, electrons are scattered
objects are magnified 1000 t 10000 times

18
Q

what are the uses for SEM?

A

detailed external features

viewing viruses

19
Q

what are the features of TEM?

A

ultrathin section of specimen are cute
section are treated with lead
beam of electrons passes through section
objects are magnified 10000 to 100000 times

20
Q

what are the uses of TEM?

A

detailed internal structures

viewing viruses

Microbiology (12 decks)

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